Abstract Indirect bioleaching of Co and Ni from iron rich laterite ore was studied, using spent media produced by four fungal and bacterial species including Pseudomonas putida, Pseudomonas koreensis, Aspergillus niger and Penicillium bilaji. HPLC analysis showed that the main carboxylic acids in spent media were gluconic, citric and oxalic acids. Gluconic acid (with 10.8–14.4 g/l) was the predominant metabolite in spent media of Aspergillus niger and Pseudomonas species, whereas citric acid (with 6.2 g/l) was the main metabolite for Penicillium bilaji. Bioleaching kinetics was assessed at 45, 75 and 90 °C. Results indicated that the highest recovery rate of Ni and Co obtained from spent medium of Pseudomonas putida were 90.6 and 71.98%, achieved at 90 °C after 3 h. The corresponding activation energy for Ni and Co solubilization using spent medium of Pseudomonas putida were 41.82 and 43.91 kJ/mol, indicating that solubilization rate of Ni and Co from iron rich laterite ore is governed by a chemically controlled reaction mechanism.

中文翻译：

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从富铁红土矿石中间接生物浸出 Co 和 Ni，使用 P. putida、P. koreensis、P. bilaji 和 A. niger 产生的代谢羧酸

摘要 使用由恶臭假单胞菌、韩国假单胞菌、黑曲霉和比拉吉青霉等四种真菌和细菌产生的废培养基，研究了从富铁红土矿石中间接生物浸出 Co 和 Ni 的情况。HPLC 分析表明废培养基中的主要羧酸是葡萄糖酸、柠檬酸和草酸。葡萄糖酸（10.8-14.4 克/升）是黑曲霉和假单胞菌用过的培养基中的主要代谢物，而柠檬酸（6.2 克/升）是比拉吉青霉的主要代谢物。在 45、75 和 90 °C 下评估了生物浸出动力学。结果表明，从恶臭假单胞菌用过的培养基中获得的 Ni 和 Co 的最高回收率为 90.6% 和 71.98%，在 90 °C 下 3 h 后达到。