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Epigallocatechin-3-gallate enhances tomato resistance to tobacco mosaic virus by modulating RBOH1-dependent H2O2 signaling.
Plant Physiology and Biochemistry ( IF 6.5 ) Pub Date : 2020-03-07 , DOI: 10.1016/j.plaphy.2020.03.008
Xue-Ning Zhang 1 , Yang-Wen-Ke Liao 2 , Xiao-Rong Wang 3 , Lan Zhang 4 , Golam Jalal Ahammed 5 , Qing-Yun Li 6 , Xin Li 4
Affiliation  

Tobacco mosaic virus (TMV) is one of the most damaging plant viruses from an economic and research point of view. Epigallocatechin-3-Gallate (EGCG), a flavonoid type secondary metabolite can selectively improve plant defense against pathogens; however, the effect of EGCG on plant defense against TMV and the underlying mechanism(s) remain elusive. In this study, exogenous EGCG application increased plant resistance to TMV as revealed by significantly decreased transcript levels of TMV-coat protein (CP) in tomato leaves. A time-course of H2O2 concentrations in tomato leaves showed that TMV inoculation rapidly increased the H2O2 accumulation, reaching its peak at 3 days post-inoculation (dpi) which remained the highest until 6 dpi. However, the combined treatment of EGCG and TMV remarkably decreased the concentrations of H2O2 at 3 and 6 dpi. Meanwhile, the transcript levels of RESPIRATORY BURST OXIDASE HOMOLOG 1 (SlRBOH1) were significantly increased by either EGCG or TMV inoculation, but the EGCG treatment along with TMV caused a further upregulation in the SlRBOH1 transcripts compared with that in only TMV-inoculated plants. Chemical scavenging of H2O2 or silencing SlRBOH1 both compromised the EGCG-induced enhanced resistance to TMV. Furthermore, EGCG-induced elevation in the activity of antioxidant enzymes was abolished by SlRBOH1 silencing, suggesting that EGCG enhanced defense against TMV by increasing the antioxidant enzyme activity via RBOH1-dependent H2O2 signaling. Taken together, our results suggest that EGCG functioned to maintain a delicate balance between ROS signaling and ROS scavenging via RBOH1, which enhanced tomato resistance to TMV.



中文翻译:

Epigallocatechin-3-gallate通过调节RBOH1依赖性H2O2信号传导增强番茄对烟草花叶病毒的抗性。

从经济和研究的角度来看,烟草花叶病毒(TMV)是最具破坏性的植物病毒之一。类黄酮型次生代谢产物Epigallocatechin-3-Gallate(EGCG)可以选择性地提高植物对病原体的防御能力;但是,EGCG对植物对抗TMV的防御作用及其潜在机制仍然难以捉摸。在这项研究中,外源EGCG的应用增加了植物对TMV的抗性,番茄叶中TMV-外壳蛋白CP)的转录水平显着降低表明了这种抗性。番茄叶片中H 2 O 2浓度随时间变化,表明TMV接种迅速增加了H 2 O 2累积,在接种后3天(dpi)达到最高,直到6 dpi才达到最高。但是,EGCG和TMV的联合处理显着降低了3 dpi和6 dpi时H 2 O 2的浓度。同时,通过EGCG或TMV接种,呼吸爆发性氧化酶同系物1(SlRBOH1)的转录水平显着增加,但是与仅TMV接种的植物相比,EGCG处理与TMV一起导致SlRBOH1转录物中的进一步上调。化学清除H 2 O 2或使SlRBOH1沉默两者都损害了EGCG诱导的对TMV的增强抗性。此外,SlRBOH1沉默消除了EGCG诱导的抗氧化酶活性的升高,表明EGCG通过增加RBOH1依赖性H 2 O 2信号传导的抗氧化酶活性,增强了对TMV的防御。两者合计,我们的结果表明EGCG的功能是维持ROS信号和通过RBOH1清除ROS之间的微妙平衡,从而增强了番茄对TMV的抗性。

更新日期:2020-03-09
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