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Molecular detection of the powdery mildew resistance genes in winter wheats DH51302 and Shimai 26
Journal of Integrative Agriculture ( IF 4.8 ) Pub Date : 2020-03-09 , DOI: 10.1016/s2095-3119(19)62644-4
Yun-feng QU , Pei-pei WU , Jing-huang HU , Yong-xing CHEN , Zhan-liang SHI , Dan QIU , Ya-hui LI , Hong-jun ZHANG , Yang ZHOU , Li YANG , Hong-wei LIU , Tong-quan ZHU , Zhi-yong LIU , Yan-ming ZHANG , Hong-jie LI

Resistance to powdery mildew is an important trait of interest in many wheat breeding programs. The information on genes conferring resistance to powdery mildew in wheat cultivars is useful in parental selection. Winter wheat breeding line DH51302 derived from Liangxing 99 and cultivar Shimai 26 derived from Jimai 22 showed identical infection patterns against 13 isolates of Blumeria graminis f. sp. tritici (Bgt) that causes wheat powdery mildew. DH51302 and Shimai 26 were crossed to a powdery mildew susceptible cultivar Zhongzuo 9504 and the F2:3 families were used in molecular localization of the resistance genes. Fourteen polymorphic markers, which were linked to Pm52 from Liangxing 99, were used to establish the genetic linkage maps for the resistance genes PmDH51302 and PmSM26 in DH51302 and Shimai 26, respectively. These genes were placed in the same genetic interval where Pm52 resides. Analysis of gene-linked molecular markers indicated that PmDH51302 and PmSM26 differed from other powdery mildew resistance genes on chromosome arm 2BL, such as Pm6, Pm33, Pm51, MlZec1, MlAB10, and Pm64. Based on the results of reaction patterns to different Bgt isolates and molecular marker localization, together with the pedigree information, DH51302 and Shimai 26 carried the same gene, Pm52, which confers their resistance to powdery mildew.



中文翻译:

冬小麦DH51302和Shimai 26的抗白粉病基因的分子检测。

抗白粉病是许多小麦育种计划中重要的重要特性。有关赋予小麦品种白粉病抗性基因的信息可用于亲本选择。来源于梁兴99的冬小麦育种系DH51302和来自集麦22的栽培品种Shimai 26表现出相同的感染模式,分别针对13种分离的Blumeria graminis f。sp。小麦白粉病的小麦Bgt)。将DH51302和Shimai 26与对白粉病敏感的品种Zhongzuo 9504杂交,并将F 2:3家族用于抗性基因的分子定位。十四个多态性标记,链接到Pm52用来自良兴99号的抗性基因分别建立DH51302Shimai 26中抗性基因PmDH51302PmSM26的遗传连锁图。这些基因被放置在Pm52所在的相同基因间隔中。基因连锁分子标记的分析表明,PmDH51302PmSM26与2BL染色体臂上的其他抗白粉病基因不同,例如Pm6,Pm33,Pm51,MlZec1,MlAB10Pm64。根据对不同Bgt分离物的反应模式的结果和分子标记的定位,再加上系谱信息,DH51302和Shimai 26携带相同的基因Pm52, 赋予它们抗白粉病的能力。

更新日期:2020-03-09
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