Ultrasonics Sonochemistry ( IF 8.4 ) Pub Date : 2020-03-09 , DOI: 10.1016/j.ultsonch.2020.105063 Zhiyu Li , Jianyi Wang , Baodong Zheng , Zebin Guo
The effects of microwave, ultrasound and combined ultrasound-microwave (UM) treatment with different intensities on structural and hydrolysis properties of myofibrillar protein (MP) were investigated. Free radical scavenging ability, angiotensin-I-converting enzyme (ACE) inhibitory activity, and cellular antioxidant and anti-inflammatory abilities of the related bioactive peptides were also evaluated. Raman spectroscopic analysis indicated that MP molecule tended to unfold and stretch with increasing in β-turn and random coil content under mild microwave (100 W), ultrasound (100−200 W) and combined UM treatments. Meanwhile, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) revealed these treatments could also improve the thermal stability against heat-induced denaturation and degeneration. The 200 W ultrasound treatment clearly increased MP solubility by disrupting the highly-ordered aggregates into smaller filament and fragment structures. The 300 W ultrasound coupled with 100 W microwave treatment further enhanced these effects. The resulting partially denatured structure induced by suitable ultrasound and combined UM treatments increased the susceptibility of MP to exogenous enzymes, thereby accelerating hydrolytic process and yielding a high peptide concentration in MP hydrolysates. MP peptides could effectively inhibit free radical and ACE activity, which also improved the ability of antioxidant defence system, and suppressed the production of proinflammatory cytokines in RAW 264.7 cells stimulated by H2O2. The combination of 100 W microwave and 300 W ultrasound treatment was optimal method for generating bioactive MP peptides with the strongest multi-activity effects against H2O2-induced cell damage.
中文翻译:
组合的超声微波处理对金色金线鱼科的结构和功能特性的影响(金线鱼)肌原纤维蛋白和水解产物
研究了不同强度的微波,超声和超声-微波联合处理对肌原纤维蛋白(MP)结构和水解性能的影响。还评估了自由基清除能力,血管紧张素-I转换酶(ACE)抑制活性以及相关生物活性肽的细胞抗氧化剂和抗炎能力。拉曼光谱分析表明,在轻度微波(100 W),超声(100-200 W)和联合UM处理下,MP分子会随着β转角和随机线圈含量的增加而展开和拉伸。同时,差示扫描量热法(DSC)和热重分析(TGA)揭示了这些处理方法还可以提高抗热诱导的变性和变性的热稳定性。200 W超声处理通过将高度有序的聚集体破碎成较小的细丝和碎片结构,明显提高了MP的溶解度。300 W超声与100 W微波处理相结合,进一步增强了这些效果。通过适当的超声和联合UM处理诱导的部分变性结构会增加MP对外源酶的敏感性,从而加速水解过程并在MP水解物中产生高肽浓度。MP肽可有效抑制自由基和ACE活性,从而提高抗氧化防御系统的能力,并抑制H刺激的RAW 264.7细胞中促炎细胞因子的产生 300 W超声与100 W微波处理相结合,进一步增强了这些效果。通过适当的超声和联合UM处理诱导的部分变性结构会增加MP对外源酶的敏感性,从而加速水解过程并在MP水解物中产生高肽浓度。MP肽可有效抑制自由基和ACE活性,从而提高抗氧化防御系统的能力,并抑制H刺激的RAW 264.7细胞中促炎细胞因子的产生 300 W超声与100 W微波处理相结合,进一步增强了这些效果。通过适当的超声和联合UM处理诱导的部分变性结构会增加MP对外源酶的敏感性,从而加速水解过程并在MP水解物中产生高肽浓度。MP肽可有效抑制自由基和ACE活性,从而提高抗氧化防御系统的能力,并抑制H刺激的RAW 264.7细胞中促炎细胞因子的产生 从而加速了水解过程,并在MP水解物中产生了高浓度的肽。MP肽可有效抑制自由基和ACE活性,从而提高抗氧化防御系统的能力,并抑制H刺激的RAW 264.7细胞中促炎细胞因子的产生 从而加速了水解过程,并在MP水解物中产生了高浓度的肽。MP肽可有效抑制自由基和ACE活性,从而提高抗氧化防御系统的能力,并抑制H刺激的RAW 264.7细胞中促炎细胞因子的产生2 O 2。100 W微波和300 W超声处理相结合是产生具有生物活性的MP肽的最佳方法,该MP肽对H 2 O 2诱导的细胞损伤具有最强的多重活性。