Hydrogels with controlled degradation and sustained bactericidal activities are promising biomaterial substrates to repair or regenerate injured tissue. In this work, we present a unique pair of lysozyme and chitosan as a hydrogel that can promote cell growth and proliferation while concomitantly preventing infection during the gradual process of hydrogel degradation and tissue ingrowth. Lysozyme- and chitosan-containing cell adhesion motifs are chemically modified with photoreactive methacrylate moieties to obtain a cross-linked hydrogel network by visible light irradiation. The resulting lysozyme–chitosan conjugate successfully modulates the degradation rate of hydrogels, while promoting cell adhesion, proliferation, and matrix formation with no cytotoxicity. The hydrogel also exerts an intrinsic antibacterial effect by combining antimicrobial features of chitosan and lysozyme. This work demonstrates an advanced hydrogel platform with the dual function of tunable degradation and infection control for tissue engineering and wound healing applications.

中文翻译：

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双重功能的溶菌酶-壳聚糖偶联物可调节降解和抗菌活性。

具有受控降解和持续杀菌活性的水凝胶是有望修复或再生受伤组织的生物材料基质。在这项工作中，我们提出了一对独特的溶菌酶和壳聚糖作为水凝胶，可以促进细胞生长和增殖，同时在水凝胶降解和组织向内生长的过程中同时防止感染。含有溶菌酶和壳聚糖的细胞粘附基序可以通过光反应性甲基丙烯酸酯部分进行化学修饰，从而通过可见光照射获得交联的水凝胶网络。产生的溶菌酶-壳聚糖偶联物成功地调节了水凝胶的降解速率，同时促进了细胞粘附，增殖和基质形成，而没有细胞毒性。通过结合壳聚糖和溶菌酶的抗菌特性，水凝胶还发挥了固有的抗菌作用。这项工作演示了先进的水凝胶平台，具有可调节的降解和感染控制双重功能，适用于组织工程和伤口愈合应用。