Lactobacillus plantarum FZU3013, a probiotic previously isolated from the traditional brewing process of Hongqu rice wine, may have the beneficial effect of improving the disorders of lipid metabolism. This study aimed to investigate the role of L. plantarum FZU3013 in improving non-alcoholic fatty liver (NAFL) associated with hyperlipidemia in mice fed a high-fat diet. The results indicated that L. plantarum FZU3013 intervention significantly reduced the HFD-induced body weight gain and the abnormal levels of serum total triglycerides (TG), total cholesterol (TC) and low-density lipoprotein (LDL-C), and inhibited the excessive accumulation of liver lipids. In addition, L. plantarum FZU3013 also promoted the excretion of bile acids through feces. Metagenomic and multivariate statistical analysis revealed that L. plantarum FZU3013 made significant structural changes in the intestinal microbiome of the mice fed with HFD, in particular by modulating the relative abundance of some function related microbial phylotypes. Furthermore, ultra-performance liquid chromatography with quadruple-time of flight mass spectrometry (UPLC-QTOF/MS)-based liver metabolomics demonstrated that L. plantarum FZU3013 had a significant regulatory effect on the composition of liver metabolites in hyperlipidemic mice, especially on the levels of some important biomarkers involved in the pathways of glycerophospholipid metabolism, fatty acid degradation, fatty acid elongation, glycerolipid metabolism, primary bile acid biosynthesis, arachidonic acid metabolism, etc. Moreover, L. plantarum FZU3013 regulated the mRNA expression levels of the genes responsible for liver lipid and cholesterol metabolism. L. plantarum FZU3013 intervention increased the hepatic mRNA levels of cholesterol 7α-hydroxylase (CYP7A1) and the bile salt export pump (BSEP), suggesting enhanced bile acid synthesis and excretion from the liver. These findings present new evidence supporting that L. plantarum FZU3013 has the potential to improve lipid metabolism disorders through modulating specific intestinal microbial phylotypes and regulating hepatic lipid metabolism related genes, therefore it could be used as a potential functional food for the prevention of NAFL and hyperlipidemia.

中文翻译：

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植物乳杆菌FZU3013对高脂饮食小鼠高脂血症相关的非酒精性脂肪肝的保护作用。

植物乳杆菌FZU3013是一种从红曲米酒的传统酿造过程中分离出来的益生菌，可能具有改善脂质代谢紊乱的有益作用。这项研究旨在研究高脂饮食小鼠中植物乳杆菌FZU3013在改善与高脂血症相关的非酒精性脂肪肝（NAFL）中的作用。结果表明，植物乳杆菌FZU3013干预可显着降低HFD诱导的体重增加，并降低血清总甘油三酸酯（TG），总胆固醇（TC）和低密度脂蛋白（LDL-C）的异常水平，并抑制过量摄入肝脂质积聚。此外，植物乳杆菌FZU3013还通过粪便促进了胆汁酸的排泄。元基因组和多元统计分析表明，L。车前草FZU3013在喂食HFD的小鼠的肠道微生物组中发生了显着的结构变化，特别是通过调节某些功能相关微生物系统型的相对丰度。此外，基于四倍飞行时间质谱（UPLC-QTOF / MS）的肝脏代谢组学的超高效液相色谱法表明，植物乳杆菌FZU3013对高脂血症小鼠的肝脏代谢物的组成具有重要的调节作用，尤其是对甘油磷脂代谢，脂肪酸降解，脂肪酸延伸，甘油脂代谢，初级胆汁酸生物合成，花生四烯酸代谢等途径中涉及的一些重要生物标志物的水平。车前草FZU3013调节负责肝脏脂质和胆固醇代谢的基因的mRNA表达水平。植物乳杆菌FZU3013干预可增加胆固醇7α-羟化酶（CYP7A1）和胆盐输出泵（BSEP）的肝mRNA水平，表明胆汁酸的合成和从肝脏的排泄增加。这些发现提供了新的证据，证明植物乳杆菌FZU3013有潜力通过调节特定的肠道微生物系统型和调节肝脂质代谢相关基因来改善脂质代谢紊乱，因此可以用作预防NAFL和高脂血症的潜在功能性食品。 。提示胆汁酸合成和从肝脏排泄增强。这些发现提供了新的证据，证明植物乳杆菌FZU3013有潜力通过调节特定的肠道微生物系统型和调节肝脂质代谢相关基因来改善脂质代谢紊乱，因此可以用作预防NAFL和高脂血症的潜在功能性食品。 。提示胆汁酸合成和从肝脏排泄增强。这些发现提供了新的证据，证明植物乳杆菌FZU3013有潜力通过调节特定的肠道微生物系统型和调节肝脂质代谢相关基因来改善脂质代谢紊乱，因此可以用作预防NAFL和高脂血症的潜在功能性食品。 。