Abstract

The corrosion behavior of a binary Co-20 Cr alloy was investigated to determine the biocompatibility and corrosion rate associated with the release of metal ions that can produce adverse effects inside the human body. Co-Cr and 316L SS alloys were corrosion tested in artificial saliva by electrochemical techniques. For biocompatibility assay, human osteoblast cells were cultured in a Dulbecco’s modified Eagle’s medium, and for understanding the osteoblast function over the surface materials, the cells were cultured in “mineralizing media.” Polarization curves indicated that the corrosion rate of 316L SS was reduced when compared with the one exhibited by the Co-Cr alloy. According to linear polarization resistance curves, the R_p values for the Co-20 Cr (M1) alloy were higher than those of the 316L SS after 9 days of immersion. This indicates that the Co-20 Cr (M1) alloy developed a more stable protective film on its surface, after the 9th day of immersion. The incubation of human osteoblasts revealed that the osteoblasts covered the entire surface of the Co-20 Cr alloy. It resulted in an outstanding cell–surface contact with a polygonal and extended morphology with filopodia and lamellipodia without any cytotoxic response.

中文翻译：

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植入式口腔钴铬合金在人工唾液中的腐蚀评估及生物相容性

摘要

研究了二元Co-20 Cr合金的腐蚀行为，以确定与金属离子释放相关的生物相容性和腐蚀速率，这些金属离子可能在人体内部产生不利影响。通过电化学技术在人工唾液中对Co-Cr和316L SS合金进行了腐蚀测试。为了进行生物相容性测定，将人类成骨细胞在Dulbecco改良的Eagle培养基中进行培养，为了了解成骨细胞在表面材料上的功能，将细胞在“矿化培养基”中进行培养。极化曲线表明，与Co-Cr合金相比，316L SS的腐蚀速率降低了。根据线性极化电阻曲线，R _p浸入9天后，Co-20 Cr（M1）合金的值高于316L SS的值。这表明在浸泡第9天后，Co-20 Cr（M1）合金在其表面上形成了更稳定的保护膜。人类成骨细胞的孵育表明，成骨细胞覆盖了Co-20 Cr合金的整个表面。它导致细胞表面具有出色的接触性，并带有丝状伪足和片状脂质体的多边形和扩展形态，而没有任何细胞毒性反应。