Characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in China.,Virology

当前位置： X-MOL 学术 › Virology › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in China.
Virology ( IF 3.7 ) Pub Date : 2020-03-06 , DOI: 10.1016/j.virol.2020.03.001
Rongli Guo ₁ , Baochao Fan ₂ , Xinjian Chang ₁ , Jinzhu Zhou ₁ , Yongxiang Zhao ₁ , Danyi Shi ₃ , Zhengyu Yu ₁ , Kongwang He ₁ , Bin Li ₄

Affiliation

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, Jiangsu, 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225000, China; Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225000, China.
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, Jiangsu, 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225000, China; Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225000, China; Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, 210014, China.
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, Jiangsu, 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225000, China; Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225000, China; College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066004, China.
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, Jiangsu, 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225000, China; Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225000, China; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.

Porcine transmissible gastroenteritis virus (TGEV) is one of the major etiological agents of viral enteritis and fetal diarrhea in suckling piglets. In this study, a TGEV JS2012 strain was isolated from the feces of piglets in Jiangsu Province, China. The phylogenetic analysis showed that TGEV JS2012 was placed between the Purdue and the Miller clusters. Analysis of recombination confirmed that TGEV JS2012 is a natural recombinant strain between Miller M6 and Purdue 115. Similar to Miller M6, virulent Purdue and China strain TS, in S gene the JS2012 maintained genetic integrity and the characteristics of the TGEV virulent strains. In vivo, TGEV JS2012 caused 100% mortality in newborn piglets, indicating the strong pathogenicity of this isolate. These results reveal that the JS2012 is a novel natural recombinant TGEV with high virulence. Our findings provide valuable information about genetic diversity and infection mechanism of the coronavirus family.

更新日期：2020-03-06

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>