We report a heterojunction Bi₂WO₆/WS_2-x with sulfur vacancies as a broad-spectrum bactericide to efficiently kill Gram-positive and Gram-negative bacteria in vitro and in vivo under visible-light irradiation. Sulfur vacancies in single-layer WS₂ make the surface electron-rich. Integration of Bi₂WO₆ with WS₂ enhances the photoelectric activity under visible-light irradiation. Sulfur vacancies promote the generation of radicals and the extraction of membrane phospholipids from bacterial cells. Density functional theory verifies that S vacancies strengthen the interactions between the Bi₂WO₆/WS_2-x surface and H₂O, enhancing the generation of ·OH. Two-dimensional correlation spectroscopy analysis reveals that perturbation of β-sheet proteins and formation of outer-sphere surface complexes contribute to the high antibacterial capacity. Bi₂WO₆/WS_2-x accelerated the re-epithelialization and healing of infected wounds in an animal model. Uncommonly, Bi₂WO₆/WS_2-x does not exhibit drug resistance and is biocompatible with human cells. Our results indicate that vacancy-functionalized heterojunctions are potentially promising antibacterial agents by regulating the interface interaction between biology and nanomaterials.

我们报告异质结Bi ₂ WO ₆ / WS _2-x与硫空位作为一种广谱杀菌剂，可有效杀死体外和体内可见光照射下的革兰氏阳性和革兰氏阴性细菌。单层WS _2中的硫空位使表面富电子。Bi ₂ WO ₆与WS _2的集成增强了可见光照射下的光电活性。硫空位促进自由基的产生和从细菌细胞中提取膜磷脂。密度泛函理论证明S空位增强了Bi ₂之间的相互作用WO ₆ / WS _2-x表面和H ₂ O，增强了·OH的生成。二维相关光谱分析表明，β-折叠蛋白的扰动和外球表面复合物的形成有助于高抗菌能力。Bi ₂ WO ₆ / WS _2-x加快了动物模型中感染伤口的再上皮化和愈合。不常见的是Bi ₂ WO ₆ / WS _2-x不显示耐药性，并且与人细胞具有生物相容性。我们的结果表明，通过调节生物学与纳米材料之间的界面相互作用，空位官能化的异质结是潜在有希望的抗菌剂。