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Hierarchical 0D-2D bio-composite film based on enzyme-loaded polymeric nanoparticles decorating graphene nanosheets as a high-performance bio-sensing platform
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2020-03-06 , DOI: 10.1016/j.bios.2020.112134
Sheng Xu , Yayuan Liu , Wei Zhao , Qian Wu , Yanru Chen , Xuewen Huang , Zhijian Sun , Ye Zhu , Xiaoya Liu

Herein, we developed a hierarchical bio-composite sensing film by facile one-step electro-deposition of 0D enzyme-polymer nanoparticles (NPs) with 2D graphene oxide nanosheets as conductive supports and nanofillers, based on which an effective and robust enzymatic biosensor platform was constructed. Horseradish peroxidase (HRP) as a model enzyme was co-assembled with a photo-cross-linkable polypeptide of 2-hydroxyethyl methacrylate modified poly(γ-glutamic acid) (γ-PGA-HEMA), generating hybrid HRP@γ-PGA-HEMA nanoparticles (HRP@PGH NPs). Then HRP@PGH NPs and graphene oxide nanosheets (GO NSs) were simultaneously electrodeposited onto the electrode surface, obtaining a hierarchical 0D-2D bio-composite film. After subsequent electrochemical reduction of GO NSs into graphene nanosheets (GNSs) and following photo-cross-linking, the resultant nanostructured HRP@PGH/GNSs sensing film was successfully applied to construct an enzymatic biosensor for hydrogen peroxide (H2O2). The biosensor exerted high sensitivity, fast response, and good stability for H2O2 sensing. Satisfactory results were also demonstrated for its practical application in human serum samples, suggesting a promising application potential in biomedical diagnostics. The one-step generated 0D-2D bio-composite sensing film demonstrates synergetic effects from both the soft nanoparticles and hard conductive nanosheets, which would enlighten the innovative construction of composite nanomaterials and nanoarchitectonics for bio-sensing systems.



中文翻译:

基于酶负载的聚合物纳米颗粒的分层0D-2D生物复合膜,装饰石墨烯纳米片,作为高性能生物传感平台

本文中,我们通过以二维氧化石墨烯纳米片作为导电载体和纳米填料的0D酶-聚合物纳米颗粒(NP)的便捷一步电沉积,开发了一种分层的生物复合传感膜,在此基础上,建立了一种有效而强大的酶促生物传感器平台建。将辣根过氧化物酶(HRP)作为模型酶与甲基丙烯酸2-羟乙酯修饰的聚(γ-谷氨酸)(γ-PGA-HEMA)的可光交联多肽共组装,生成杂合HRP @γ-PGA- HEMA纳米粒子(HRP @ PGH NP)。然后将HRP @ PGH NP和氧化石墨烯纳米片(GO NSs)同时电沉积到电极表面上,得到分层的0D-2D生物复合膜。随后将GO NSs电化学还原为石墨烯纳米片(GNSs),并进行光交联后,2 O 2)。该生物传感器对H 2 O 2的传感具有高灵敏度,快速响应和良好的稳定性。还证明了其在人血清样品中的实际应用具有令人满意的结果,表明在生物医学诊断中有广阔的应用前景。一步生成的0D-2D生物复合传感膜展示了软纳米颗粒和硬导电纳米片的协同效应,这将启发用于生物传感系统的复合纳米材料和纳米建筑学的创新构造。

更新日期:2020-03-06
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