Conformable, wearable biosensor-integrated systems are a promising approach to non-invasive and quantitative on-body detection of biomarkers in body fluids. However, realizing such a system has been slowed by the difficulty of fabricating a soft affinity-based biosensor patch capable of precise on-body fluid handling with minimal wearer intervention and a simple measurement protocol. Herein, we demonstrate a conformable, wearable lab-on-a-patch (LOP) platform composed of a stretchable, label-free, impedimetric biosensor and a stretchable microfluidic device for on-body detection of the hormone biomarker, cortisol. The all-in-one, stretchable microfluidic device can precisely collect and deliver sweat for cortisol quantitation and offers one-touch operation of reagent delivery for simultaneous electrochemical signal generation and washing. Three-dimensional nanostructuring of the Au working electrode enables the high sensitivity required to detect the pM-levels of cortisol in sweat. Our integrated LOP detected sweat cortisol quantitatively and accurately during exercise. This LOP will open a new horizon for non-invasive, highly sensitive, and quantitative on-body immunodetection for wearable personal diagnostics.

整合，可穿戴的生物传感器集成系统是一种有前途的方法，可用于对体液中生物标志物进行无创且定量的人体检测。然而，由于难以制造能够以最小的佩戴者干预和简单的测量方案来精确地进行体液处理的基于软亲和力的生物传感器贴片，使实现这种系统的速度变慢。在这里，我们展示了一个可整合的，可穿戴的补丁实验室（LOP）平台，该平台由可拉伸，无标签的阻抗生物传感器和可拉伸的微流体装置组成，可用于体内检测激素生物标志物皮质醇。多合一的可拉伸微流控设备可以精确收集和输送汗液以进行皮质醇定量分析，并提供一键式试剂输送操作，可同时生成和洗涤电化学信号。Au工作电极的三维纳米结构可实现检测汗液中皮质醇的pM水平所需的高灵敏度。我们集成的LOP在运动过程中可以定量，准确地检测出汗皮质醇。该LOP将为可穿戴式个人诊断程序的非侵入性，高度灵敏和定量的人体免疫检测开辟新的视野。