BACKGROUND Human papillomavirus (HPV) infection is a primary cause of cervical cancer. Although epidemiologic study revealed that carcinogenic risk differs according to HPV genotypes, the expression patterns of HPV-derived transcripts and their dependence on HPV genotypes have not yet been fully elucidated. METHODS In this study, 382 patients with abnormal cervical cytology were enrolled to assess the associations between HPV-derived transcripts and cervical intraepithelial neoplasia (CIN) grades and/or HPV genotypes. Specifically, four HPV-derived transcripts, namely, oncogenes E6 and E6*, E1^E4, and viral capsid protein L1 in four major HPV genotypes-HPV 16, 18, 52, and 58-were investigated. RESULTS The detection rate of E6/E6* increased with CIN progression, whereas there was no significant change in the detection rate of E1^E4 or L1 among CIN grades. In addition, we found that L1 gene expression was HPV type-dependent. Almost all HPV 52-positive specimens, approximately 50% of HPV 58-positive specimens, around 33% of HPV 16-positive specimens, and only one HPV18-positive specimen expressed L1. CONCLUSIONS We demonstrated that HPV-derived transcripts are HPV genotype-dependent. Especially, expression patterns of L1 gene expression might reflect HPV genotype-dependent patterns of carcinogenesis.

中文翻译：

---

人乳头瘤病毒16、18、52和58来源的转录本在宫颈上皮内瘤变中的差异表达。

背景技术人乳头瘤病毒（HPV）感染是子宫颈癌的主要原因。尽管流行病学研究表明，根据HPV基因型的不同，致癌风险有所不同，但尚未完全阐明HPV衍生转录本的表达方式及其对HPV基因型的依赖性。方法该研究招募了382例宫颈细胞学异常的患者，以评估其HPV转录本与宫颈上皮内瘤变（CIN）等级和/或HPV基因型之间的关联。具体地，研究了四种主要HPV基因型-HPV 16、18、52和58中的四种HPV衍生的转录本，即癌基因E6和E6 *，E1 ^ E4和病毒衣壳蛋白L1。结果E6 / E6 *的检出率随CIN进展而增加，而在CIN等级中，E1 ^ E4或L1的检出率无明显变化。此外，我们发现L1基因表达是HPV类型依赖性的。几乎所有HPV 52阳性标本，约50％的HPV 58阳性标本，约33％的HPV 16阳性标本，只有1个HPV18阳性标本表达L1。结论我们证明HPV衍生的转录本是HPV基因型依赖性的。特别是，L1基因表达的表达模式可能反映了HPV基因型依赖的致癌模式。结论我们证明HPV衍生的转录本是HPV基因型依赖性的。特别是，L1基因表达的表达模式可能反映了HPV基因型依赖的致癌模式。结论我们证明HPV衍生的转录本是HPV基因型依赖性的。特别是，L1基因表达的表达模式可能反映了HPV基因型依赖的致癌模式。