BACKGROUND Emerging arboviral diseases like Zika, dengue and chikungunya that are transmitted by Aedes aegypti mosquitoes, are increasingly threatening human health. Blends of human-like synthetic chemical attractants can be used to attract host-seeking mosquitoes. The aim of this study was to test new combinations of traps and odour baits in the laboratory, followed by testing the best candidates in the field to improve Ae. aegypti monitoring and surveillance. METHODS First, the BG-Suna trap was evaluated for capturing laboratory-reared Ae. aegypti by testing normal and inverted positions in screen cage tests. Secondly, the attractiveness of the MB5 blend, CO2, and their combination was tested. Thirdly, we tested the attractiveness of different trap types (BG-Suna, BG-Sentinel, MM-X and CDC light trap). Finally, we confirmed laboratory results in the field in Paramaribo, Suriname, using the MB5 and BG-Lure odour blends, CO2 and the BG-Sentinel and BG-Bowl trap using a Latin Square design. RESULTS The MB5 blend in combination with CO2 outperformed traps baited only with CO2 or MB5 in screen cage tests (P < 0.0001). The BG-Sentinel trap performed equally well as the inverted BG-Suna and was taken to the field (P = 0.729). In the field, we captured Ae. aegypti, Cx. quinquefasciatus and Cx. nigripalpus. We confirmed the laboratory results and found that the combination of the MB5 blend and CO2 almost doubled Ae. aegypti female captures (P = 0.004) and more than doubled Culex spp. female captures (P = 0.005) compared to using only CO2. Interestingly, the MB5 blend outperformed the commercially available BG-Lure, in the BG-Sentinel (P < 0.001). The BG-Bowl also attracted Ae. aegypti when baited with the MB5 blend in similar numbers as the BG-Sentinel baited with the MB5 (P = 0.362). CONCLUSIONS Our study demonstrated that the BG-Sentinel trap baited with the MB5 blend and CO2 outperforms the current golden standard (BG-Sentinel trap with BG-Lure) for monitoring Ae. aegypti females and males, in both laboratory and field experiments. The BG-Bowl baited with the MB5 blend is a good candidate for home use. Finally, the results show that CO2 is an indispensable component of the attractive blend.

中文翻译：

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苏里南帕拉马里博市诱蚊诱捕装置的优化和现场验证。

背景技术埃及伊蚊（Aedes aegypti蚊子）传播的诸如寨卡病毒（Zika），登革热和基孔肯雅热等新型虫媒病毒疾病正日益威胁着人类健康。类似人的合成化学引诱剂的混合物可用于吸引寻求宿主的蚊子。这项研究的目的是在实验室中测试陷阱和臭味诱饵的新组合，然后测试该领域的最佳人选以改善Ae。埃及监测和监视。方法首先，对BG-Suna阱进行了评估，以捕获实验室饲养的Ae。通过在筛笼测试中测试正常和倒立位置来实现埃及埃及。其次，测试了MB5共混物，CO2及其组合的吸引力。第三，我们测试了不同陷阱类型（BG-Suna，BG-Sentinel，MM-X和CDC光线陷阱）的吸引力。最后，我们使用MB5和BG-Lure气味混合物，CO2以及BG-Sentinel和BG-Bowl捕集阱采用拉丁广场设计，在苏里南帕拉马里博确认了实验室结果。结果在筛笼测试中，MB5混合物与CO2的结合性能优于仅用CO2或MB5诱集的捕集阱（P <0.0001）。BG-Sentinel捕集阱的性能与倒置的BG-Suna一样好，并被带到野外（P = 0.729）。在野外，我们捕获了Ae。埃及，Cx。quinquefasciatus和Cx。黑人。我们证实了实验室结果，发现MB5混合物和CO2的组合几乎使Ae翻了一番。埃及雌性捕获量（P = 0.004）和两倍多的库克斯菌种。与仅使用CO2相比，女性捕获量（P = 0.005）。有趣的是，MB5共混物在BG-Sentinel中的表现优于市售的BG-Lure（P <0.001）。BG-Bowl也吸引了Ae。用MB5诱饵时，埃及盾与用MB5诱饵的BG-哨兵的数量相似（P = 0.362）。结论我们的研究表明，用MB5混合物和CO2诱饵的BG-Sentinel捕集阱性能优于目前监测Ae的黄金标准（BG-Sentinel捕集阱和BG-Lure）。在实验室和野外实验中，埃及雌性和雄性。用MB5混合物诱饵的BG碗是家庭使用的不错选择。最后，结果表明，CO 2是有吸引力的共混物中必不可少的成分。结论我们的研究表明，用MB5混合物和CO2诱饵的BG-Sentinel捕集阱性能优于目前监测Ae的黄金标准（BG-Sentinel捕集阱和BG-Lure）。在实验室和野外实验中，埃及雌性和雄性。用MB5混合物诱饵的BG碗是家庭使用的不错选择。最后，结果表明，CO 2是有吸引力的共混物中必不可少的成分。结论我们的研究表明，用MB5混合物和CO2诱饵的BG-Sentinel捕集阱性能优于目前监测Ae的黄金标准（BG-Sentinel捕集阱和BG-Lure）。在实验室和野外实验中，埃及雌性和雄性。用MB5混合物诱饵的BG碗是家庭使用的不错选择。最后，结果表明，CO 2是有吸引力的共混物中必不可少的成分。