B7-H6 has been revealed as an endogenous immunoligand expressed in a variety of tumors, but not expressed in healthy tissues. Heretofore, no studies have been reported describing B7-H6 in women with cervical cancer. To investigate this question, our present study was conducted. This retrospective study comprised a total of 62 paraffinized cervical biopsies, which were distributed in five groups: low-grade squamous intraepithelial lesions (LSIL), high-grade squamous intraepithelial lesions (HSIL), squamous cervical carcinoma (SCC), uterine cervical adenocarcinoma (UCAC), and a group of cervicitis (as a control for non-abnormal/non-transformed cells). Cervical sections were stained by immunohistochemistry to explore the expression of B7-H6, which was reported according to the immunoreactive score (IRS) system. We observed a complete lack of B7-H6 in LSIL abnormal epithelial cells. Interestingly, B7-H6 began to be seen in HSIL abnormal epithelial cells; more than half of this group had B7-H6 positive cells, with staining characterized by a cytoplasmic and membranous pattern. B7-H6 in the SCC group was also seen in the majority of the sections, showing the same cytoplasmic and membranous pattern. Strong evidence of B7-H6 was notably found in UCAC tumor columnar cells (in 100% of the specimens, also with cytoplasmic and membranous pattern). Moreover, consistent B7-H6 staining was observed in infiltrating plasma cells in all groups. B7-H6 IRS positively correlated with disease stage in the development of cervical cancer; additionally, B7-H6 scores were found to be even higher in the more aggressive uterine cervical adenocarcinoma, suggesting a possible future therapeutic target for this cancer type.

中文翻译：

---

异常/转化的角质形成细胞中免疫配体B7-H6的阳性染色始终伴随宫颈癌的进展

B7-H6已被揭示为在多种肿瘤中表达但在健康组织中未表达的内源性免疫配体。迄今为止，还没有报道描述描述宫颈癌女性中B7-H6的研究。为了调查这个问题，我们进行了本研究。这项回顾性研究总共包括62例石蜡宫颈活检，分为5组：低度鳞状上皮内病变（LSIL），高度鳞状上皮内病变（HSIL），鳞状宫颈癌（SCC），子宫宫颈腺癌（ UCAC）和一组宫颈炎（作为非异常/非转化细胞的对照）。通过免疫组织化学对宫颈切片进行染色，以探讨B7-H6的表达，这是根据免疫反应评分（IRS）系统报道的。我们观察到LSIL异常上皮细胞完全缺乏B7-H6。有趣的是，在HSIL异常上皮细胞中开始出现B7-H6。该组中有一半以上具有B7-H6阳性细胞，其染色具有细胞质和膜性模式。在大多数切片中也可见到SCC组中的B7-H6，显示出相同的细胞质和膜性模式。在UCAC肿瘤柱状细胞中发现了B7-H6的有力证据（在100％的标本中，还具有细胞质和膜性模式）。此外，在所有组的浸润浆细胞中观察到一致的B7-H6染色。B7-H6国税局与子宫颈癌发展的疾病阶段呈正相关；此外，在更具侵略性的子宫宫颈腺癌中，发现B7-H6得分更高，