Previous phylogenetic analyses of species within the genus Golovinomyces (Ascomycota, Erysiphales), based on ITS and 28S rDNA sequence data, revealed a co-evolutionary relationship between powdery mildew species and hosts of certain tribes of the plant family Asteraceae. Golovinomyces growing on host plants belonging to the Heliantheae formed a single lineage, comprised of a morphologically differentiated complex of species, which included G. ambrosiae, G. circumfusus, and G. spadiceus. However, the lineage also encompassed sequences retrieved from Golovinomyces specimens on other Asteraceae tribes as well as other plant families, suggesting the involvement of a plurivorous species. A multilocus phylogenetic examination of this complex, using ITS, 28S, IGS (intergenic spacer), TUB2 (beta-tubulin), and CHS1 (chitin synthase I) sequence data was carried out to clarify the discrepancies between ITS and 28S rDNA sequence data and morphological differences. Furthermore, the circumscription of species and their host ranges were emended. The phylogenetic and morphological analyses conducted in this study revealed three distinct species named, viz., (1) G. ambrosiae emend. (including G. spadiceus), a plurivorous species that occurs on a multitude of hosts including, Ambrosia spp., multiple species of the Heliantheae and plant species of other tribes of Asteraceae including the Asian species of Eupatorium; (2) G. latisporus comb. nov. (≡ Oidium latisporum), the closely related, but morphologically distinct species confined to hosts of the Heliantheae genera Helianthus, Zinnia, and most likely Rudbeckia; and (3) G. circumfusus confined to Eupatorium cannabinum in Europe. The present results provide strong evidence that the combination of multi-locus phylogeny and morphological analysis is an effective way to identify species in the genus Golovinomyces.

中文翻译：

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Golovinomyces（Ascomycota，Erysiphales）属中尚未解决的异质物种复合体的多基因位系统发育和分类学，包括丁香假单胞菌，环抱假单胞菌和斜纹夜蛾。

根据ITS和28S rDNA序列数据，对Golovinomyces属（Ascomycota，Erysiphales）内物种的先前系统发育分析表明，白粉病菌种与菊科植物家族某些部落的宿主之间存在共同进化关系。在属于向日葵科的寄主植物上生长的戈氏菌形成了一个单一的谱系，该谱系由形态上分化的物种复合体组成，其中包括安布罗希菌，环柏和环孢菌。但是，该谱系还包含从其他菊科部落以及其他植物科的Golovinomyces标本中检索到的序列，这表明其中涉及了一个多食性物种。使用ITS，28S，IGS（基因间隔子），TUB2（β微管蛋白）对该复合体进行多基因系统发育检查，并使用CHS1（几丁质合酶I）序列数据来阐明ITS和28S rDNA序列数据之间的差异和形态差异。此外，修改了物种的界限及其寄主范围。在这项研究中进行的系统发育和形态学分析揭示了三个不同的物种，即（1）甘草假单胞菌。（包括西班牙念珠菌），是在许多寄主上出现的多食物种，包括Ambrosia spp。，Heliantheae的多种物种以及菊科的其他部落的植物物种，包括亚洲的Eupatorium；（2）G. latisporus梳子。十一月 （≡Odisium latisporum），密切相关，但形态上截然不同的物种，被限制在向日葵，百日菊属和最有可能的黄金菊的宿主中；和（3）G。circumfusus局限于欧洲的Eupatorium canabinum。目前的结果提供了有力的证据，证明多位点系统发育和形态学分析相结合是鉴定戈洛维氏菌属物种的有效方法。