BACKGROUND "Bairihua", a variety of the Catalpa bungei, has a large amount of flowers and a long flowering period which make it an excellent material for flowering researches in trees. SPL is one of the hub genes that regulate both flowering transition and development. RESULTS SPL homologues CbuSPL9 was cloned using degenerate primers with RACE. Expression studies during flowering transition in "Bairihua" and ectopic expression in Arabidopsis showed that CbuSPL9 was functional similarly with its Arabidopsis homologues. In the next step, we used Y2H to identify the proteins that could interact with CbuSPL9. HMGA, an architectural transcriptional factor, was identified and cloned for further research. BiFC and BLI showed that CbuSPL9 could form a heterodimer with CbuHMGA in the nucleus. The expression analysis showed that CbuHMGA had a similar expression trend to that of CbuSPL9 during flowering in "Bairihua". Intriguingly, ectopic expression of CbuHMGA in Arabidopsis would lead to aberrant flowers, but did not effect flowering time. CONCLUSIONS Our results implied a novel pathway that CbuSPL9 regulated flowering development, but not flowering transition, with the participation of CbuHMGA. Further investments need to be done to verify the details of this pathway.

中文翻译：

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在al蒲中开花期间，CbuSPL的潜在功能和编码其相互作用蛋白的基因。

背景技术“ Bairihua”是各种Catalpa bungei，具有大量的花和长的开花期，这使其成为用于树木的开花研究的极好的材料。SPL是调节开花过渡和发育的中枢基因之一。结果使用具有RACE的简并引物克隆了SPL同源物CbuSPL9。在“ Bairihua”开花过渡期和拟南芥中异位表达期间的表达研究表明，CbuSPL9的功能与其拟南芥同源物相似。在下一步中，我们使用Y2H识别可以与CbuSPL9相互作用的蛋白质。HMGA是一种建筑转录因子，已被鉴定并克隆用于进一步研究。BiFC和​​BLI显示CbuSPL9可以与CbuHMGA在细胞核中形成异二聚体。表达分析表明，在“百里花”开花期间，CbuHMGA具有与CbuSPL9相似的表达趋势。有趣的是，CbuHMGA在拟南芥中的异位表达将导致花异常，但不影响开花时间。结论我们的结果暗示了CbuSPL9在CbuHMGA的参与下调节开花发育而不是开花过渡的新途径。需要做进一步的投资以验证该途径的细节。在CbuHMGA的参与下。需要做进一步的投资以验证该途径的细节。在CbuHMGA的参与下。需要做进一步的投资以验证该途径的细节。