Extracellular matrix (ECM) properties affect multiple cellular processes such as cell survival, proliferation, and protein synthesis. Thus, a polymeric-cell delivery system with the ability to manipulate the extracellular environment can act as a fundamental regulator of cell function. Given the promise of stem cell therapeutics, a method to uniformly enhance stem cell function, in particular trophic factor release, can prove transformative in improving efficacy and increasing feasibility by reducing the total number of cells required. Herein, a click-chemistry powered 3D, single-cell encapsulation method aimed at synthesizing a polymeric coating with the optimal thickness around neural progenitor cells is introduced. Polymer encapsulation of neural stem cells significantly increases the release of neurotrophic factors such as VEGF and CNTF. Cell encapsulation with a soft extracellular polymer upregulates the ADCY8-cAMP pathway, suggesting a mechanism for the increase in paracrine factors. Hence, the described single-cell encapsulation technique can emerge as a translatable, nonviral cell modulation method and has the potential to improve stem cells' therapeutic effect.

中文翻译：

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通过点击化学进行的单细胞封装改变了神经祖细胞旁分泌因子的产生。

细胞外基质 (ECM) 特性影响多种细胞过程，例如细胞存活、增殖和蛋白质合成。因此，具有操纵细胞外环境能力的聚合物细胞递送系统可以作为细胞功能的基本调节剂。鉴于干细胞疗法的前景，一种统一增强干细胞功能，特别是营养因子释放的方法，可以通过减少所需的细胞总数来证明在提高疗效和增加可行性方面具有变革性。在此，介绍了一种点击化学驱动的 3D 单细胞封装方法，旨在在神经祖细胞周围合成具有最佳厚度的聚合物涂层。神经干细胞的聚合物封装显着增加了神经营养因子如 VEGF 和 CNTF 的释放。用柔软的细胞外聚合物包裹细胞可上调 ADCY8-cAMP 通路，表明旁分泌因子增加的机制。因此，所描述的单细胞封装技术可以作为一种可翻译的非病毒细胞调节方法出现，并有可能提高干细胞的治疗效果。