Research on the 3D culturing of cancer cells that better mimic in vivo solid tumors is important for efficient drug screening. Herein, a new platform that effectively facilitates the formation of cancer spheroids for anticancer drug screening is reported. Cytophilic graphene oxide (GO), when selectively coated on the sidewalls of micro‐wells fabricated from a cell‐adhesion‐resistive polymer, is found to efficiently initiates distinct donut‐like formation of cancer cell spheroids. Scanning electron microscopy and Raman mapping are used to analyze vertically coated GO micropatterns (vGO‐MPs) of different sizes (100–250 µm) on polymer platforms, and human liver cancer cells (HepG2), as a model cancer, are seeded on these platforms. Remarkably, the 150 µm‐sized platform is found to easily and rapidly generate 3D spheroids in the absence of cell‐adhesion proteins. In addition, owing to the unique characteristics of GO, vGO‐MPs are highly stable for long periods of time (≈1 month), even under harsh conditions (>70 °C). Moreover, the anticancer effects of two drugs (hydroxyurea and cisplatin) and the potential anticancer compound (curcumin) on HepG2 cells are demonstrated by simply measuring decreases in spheroid sizes. Hence, this new platform is highly promising as a cancer spheroid‐forming material for rapid drug screening.

中文翻译：

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垂直涂覆的氧化石墨烯微孔阵列用于高效癌症球体形成和药物筛选。

更好地模拟体内实体瘤的癌细胞3D培养研究对于有效的药物筛选非常重要。在此，报道了一种新的平台，该平台有效地促进了用于抗癌药物筛选的癌症球体的形成。嗜细胞石墨烯氧化物（GO）选择性地涂覆在由具有抗细胞粘附性的聚合物制成的微孔的侧壁上，可有效引发癌细胞球体的明显甜甜圈状形成。扫描电子显微镜和拉曼作图用于分析在聚合物平台上不同尺寸（100-250 µm）的垂直涂覆的GO微观图案（vGO-MPs），并将作为模型癌症的人类肝癌细胞（HepG2）植入这些细胞中平台。值得注意的是 发现150 µm大小的平台可以在没有细胞粘附蛋白的情况下轻松快速地生成3D球体。此外，由于GO的独特特性，即使在恶劣条件下（> 70°C），vGO-MP仍能长期稳定（约1个月）。此外，两种药物（羟基脲和顺铂）和潜在的抗癌化合物（姜黄素）对HepG2细胞的抗癌作用仅通过测量球体尺寸的减小即可证明。因此，该新平台作为用于快速药物筛查的癌症小球形成材料非常有前途。仅通过测量球体尺寸的减小即可证明两种药物（羟基脲和顺铂）和潜在的抗癌化合物（姜黄素）对HepG2细胞的抗癌作用。因此，该新平台作为用于快速药物筛查的癌症小球形成材料非常有前途。仅通过测量球体尺寸的减小即可证明两种药物（羟基脲和顺铂）和潜在的抗癌化合物（姜黄素）对HepG2细胞的抗癌作用。因此，该新平台作为用于快速药物筛查的癌症小球形成材料非常有前途。