Embedded viral barcoding in combination with high-throughput sequencing is a powerful technology with which to track single-cell clones. It can provide clonal-level insights into cellular proliferation, development, differentiation, migration, and treatment efficacy. Here, we present a detailed protocol for a viral barcoding procedure that includes the creation of barcode libraries, the viral delivery of barcodes, the recovery of barcodes, and the computational analysis of barcode sequencing data. The entire procedure can be completed within a few weeks. This barcoding method requires cells to be susceptible to viral transduction. It provides high sensitivity and throughput, and enables precise quantification of cellular progeny. It is cost efficient and does not require any advanced skills. It can also be easily adapted to many types of applications, including both in vitro and in vivo experiments.

中文翻译：

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使用嵌入式病毒条形码和高通量测序的克隆跟踪。

嵌入式病毒条形码与高通量测序相结合是一种强大的技术，可用于跟踪单细胞克隆。它可以提供对细胞增殖、发育、分化、迁移和治疗效果的克隆水平洞察。在这里，我们提出了病毒条形码程序的详细协议，包括条形码库的创建、条形码的病毒传递、条形码的恢复以及条形码排序数据的计算分析。整个过程可以在几周内完成。这种条形码方法要求细胞对病毒转导敏感。它提供高灵敏度和通量，并能够对细胞后代进行精确量化。它具有成本效益，不需要任何高级技能。