Abstract The application of salting-out assisted liquid–liquid extraction (SALLE) is proposed for the first time for the extraction of drugs directly from whole blood samples prior to their analysis by capillary electrophoresis or liquid chromatography coupled to UV detection. Four anticancer drugs, i.e. tyrosine kinase inhibitors (TKIs), were selected as proof-of-concept analytes. First, blood samples containing TKIs were mixed with acetonitrile in appropriate volumes to precipitate proteins. After swirling and centrifugation, sodium chloride was added to the blood-ACN mixture to induce a two phases separation. TKIs were efficiently extracted in the upper mostly organic phase which was directly analyzed by CE-UV or LC-UV. SALLE-CE-UV and SALLE-LC-UV methodologies offer many advantages by combining fast extraction procedure and separation efficiency in a simple cost-effective way. Performances of both methodologies were evaluated in terms of extraction efficiency, LOQ, LOD, specificity, repeatability, total analysis time and cost per analysis.

中文翻译：

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从人血中直接盐析辅助液-液萃取 (SALLE)：在酪氨酸激酶抑制剂分析中的应用

摘要 首次提出应用盐析辅助液-液萃取（SALLE）直接从全血样品中提取药物，然后再通过毛细管电泳或液相色谱结合紫外检测进行分析。四种抗癌药物，即酪氨酸激酶抑制剂 (TKIs)，被选为概念验证分析物。首先，将含有 TKI 的血样与适当体积的乙腈混合以沉淀蛋白质。旋转和离心后，将氯化钠加入血液-乙腈混合物中以引起两相分离。TKI 在上层主要有机相中被有效萃取，通过 CE-UV 或 LC-UV 直接分析。SALLE-CE-UV 和 SALLE-LC-UV 方法以简单经济的方式将快速提取过程和分离效率相结合，提供了许多优势。在提取效率、LOQ、LOD、特异性、可重复性、总分析时间和每次分析成本方面对两种方法的性能进行了评估。