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Improved production of echinenone and canthaxanthin in transgenic Nostoc sp. PCC 7120 overexpressing a heterologous crtO gene from Nostoc flagelliforme.
Microbiological Research ( IF 6.7 ) Pub Date : 2020-03-05 , DOI: 10.1016/j.micres.2020.126455
Xiang Gao 1 , Haiyan Xu 2 , Zhaoxia Zhu 2 , Yang She 3 , Shuifeng Ye 4
Affiliation  

Echinenone and canthaxanthin are important carotenoid pigments with food and industrial applications. Biosynthesis of echinenone and/or canthaxanthin is catalyzed by β-carotene ketolase (CrtO), with β-carotene as the substrate. In this study, we generated transgenic Nostoc sp. PCC 7120 overexpressing a heterologous crtO gene from Nostoc flagelliforme and evaluated the productivity of both pigments. Normal (BG11 medium, 30 °C) and osmotic stress (BG11 medium supplemented with 0.4 M mannitol, 30 °C) conditions were used for cultivation. As compared to control strain, production of echinenone and canthaxanthin in transgenic strain were respectively increased by more than 16 % and 80 %, under either normal or osmotic stress conditions. Especially upon the stress condition, higher proportion of echinenone and canthaxanthin in total pigments was achieved, which should be beneficial for downstream separation and purification. In addition, transgenic strain showed drought tolerance and could revive from desiccation treatment after rewetting. Thus, this study provided technical clues for production of both pigments in engineered cyanobacteria as well as for cyanobacterial anhydrobiotic engineering.



中文翻译:

转基因Nostoc sp。中海胆烯酮和角黄素的产量提高。PCC 7120过表达来自Nostoc鞭毛的异源crtO基因。

chin烯酮和角黄素是在食品和工业应用中重要的类胡萝卜素颜料。以β-胡萝卜素为底物,通过β-胡萝卜素酮醇酶(CrtO)催化海胆烯酮和/或角黄素的生物合成。在这项研究中,我们产生了转基因Nostoc sp。PCC 7120过表达来自Nostoc鞭毛的异源crtO基因并评估了两种颜料的生产率。培养使用正常(BG11培养基,30°C)和渗透压(补充有0.4 M甘露醇的BG11培养基,30°C)条件。与对照菌株相比,在正常或渗透胁迫条件下,转基因菌株中海胆烯酮和角黄素的产量分别增加了16%和80%以上。特别是在压力条件下,在总色素中海胆烯酮和角黄素的比例更高,这对下游分离和纯化应是有利的。另外,转基因菌株显示出耐旱性,并且在重新润湿后可以从干燥处理中恢复。因此,这项研究为工程蓝细菌中两种色素的生产以及蓝细菌水生生物工程提供了技术线索。

更新日期:2020-03-05
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