Macroalgae represent valuable sources of functional ingredients for fish diets, and the influence of supplemented aquafeeds on growth performance has been studied for some fish and seaweed species. In the present work, the potential immunomodulation exerted by U. ohnoi (5%) as dietary ingredient was investigated in Senegalese sole. After feeding with the experimental diets for 90 d, fish immune response before and after challenge with Photobacterium damselae subsp. piscicida (Phdp) was assessed. In absence of infection, systemic immune response was not modified by 5% U. ohnoi dietary inclusion for 90 d. Thus, no differences in liver and head kidney immune gene transcription or serum lysozyme, peroxidase, antiprotease and complement activities were observed based on the diet received by Senegalese sole specimens. Regarding mucosal immune parameters, no changes in gene transcription were detected in the skin and gills, whilst only tnf, cd4 and cd8 were significantly up-regulated in the intestine of fish fed with U. ohnoi, compared to the values obtained with control diet. On the contrary, when S. senegalensis specimens were challenged with Phdp, modulation of the immune response consisting in increased transcription of genes encoding complement (c1q4, c3, c9), lysozyme g (lysg), tumor necrosis factor alpha (tnfα) as well as those involved in the antioxidant response (gpx, sodmn) and iron metabolism (ferrm, hamp-1) was observed in the liver of fish fed with U. ohnoi. In parallel, decreased inflammatory cytokine and complement encoding gene transcription was displayed by the spleen of fish receiving the algal diet. Though mortality rates due to Phdp challenge were not affected by the diet received, lower pathogen loads were detected in the liver of soles receiving U. ohnoi diet. Further research to investigate the effects of higher inclusion levels of this seaweed in fish diets, feeding during short periods as wells as to assess the response against other pathogens needs to be carried out.

大型藻类是鱼类日粮中功能性成分的重要来源，对于某些鱼类和海藻种类，已研究了补充水产饲料对生长性能的影响。在目前的工作中，在塞内加尔鞋底中研究了作为食物成分的大头鱼（5％）所发挥的潜在免疫调节作用。用实验饮食喂养90 d后，用damselae亚种光子菌攻击前后的鱼免疫反应。评估了piscicida（Phdp）。在没有感染的情况下，5％U. ohnoi不会改变全身免疫反应膳食包容90 d。因此，根据塞内加尔唯一标本的饮食，未观察到肝和头肾免疫基因转录或血清溶菌酶，过氧化物酶，抗蛋白酶和补体活性的差异。关于粘膜免疫参数，在皮肤和g中未检测到基因转录的变化，而饲喂U.ohnoi的鱼的肠中仅tnf，cd4和cd8显着高于对照饮食。相反，当塞内加尔葡萄球菌样品受到Phdp攻击时，免疫应答的调节包括编码补体（在肝脏的肝脏中观察到了c1q4，c3，c9），溶菌酶g（lysg），肿瘤坏死因子α（tnfα）以及参与抗氧化剂反应（gpx，sodmn）和铁代谢（ferrm，hamp- 1）的那些。用大野U鱼喂养的鱼。同时，接受藻类饮食的鱼的脾脏显示出炎症细胞因子和编码补体的基因转录减少。尽管受Phdp挑战导致的死亡率不受所接受饮食的影响，但在接受U.ohnoi的鞋底肝脏中发现了较低的病原体负荷饮食。有必要进行进一步的研究，以调查这种海藻在鱼饲料中的含量较高，短期进食以及评估对其他病原体的反应的影响。