Preparation of biochar from industrial solid wastes is receiving increasing attention in recent years. In this paper, alkaline protease, neutral protease and collagenolytic protease are used in preliminary steps of leather production, which are expected to replace the traditional chemical agents while preserving quality of the finished leather. The protease application has remarkable positive influence towards characteristics of biochar prepared by collagen-containing solid wastes produced in preliminary steps. The enzymatic action time should be more than 3 h for complete permeation and catalysis, and the diameters of treated collagen fibers were in the range of 10 to 20 nm. The micro-cracks occurring on collagen fibers would have an obviously impact on the formation of biochar. The application of proteases reduce the pollution of traditional production through replacing traditional polluted chemicals, and the characteristics of biochars are obviously improved with good surface area and high carbon content approximately 70%. Its surface area can reach 967 m²/g. These biochars contain oxygen-containing functional groups, and the oxygen content of biochars are all over 20%. The enzyme application in leather production are effective to the properties of biochars prepared by collagen-containing solid wastes. This research can serve as a basis for the preparation of biochar derived from of natural bio-wastes thereby promoting the development of biomaterials.

近年来，从工业固体废物制备生物炭受到越来越多的关注。在本文中，碱性蛋白酶，中性蛋白酶和胶原蛋白酶被用于皮革生产的初步步骤，有望在保持成品皮革质量的同时取代传统的化学试剂。蛋白酶的应用对在初步步骤中产生的含胶原固体废物制备的生物炭的特性具有显着的积极影响。为了完全渗透和催化，酶促作用时间应超过3小时，并且处理过的胶原纤维的直径在10至20 nm的范围内。胶原纤维上发生的微裂纹将对生物炭的形成产生明显影响。蛋白酶的应用通过替代传统的污染化学物质，减少了传统产品的污染，生物炭的特性得到了明显的改善，具有良好的表面积和约70％的高碳含量。它的表面积可以达到967 m² /克。这些生物炭含有含氧官能团，并且生物炭的氧含量都超过20％。在皮革生产中使用酶对由含胶原的固体废物制备的生物炭的性质有效。该研究可作为制备源自天然生物废料的生物炭的基础，从而促进生物材料的发展。