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Mutation of Arthrospira platensis by gamma irradiation to promote phenol tolerance and CO2 fixation for coal-chemical flue gas reduction
Journal of CO2 Utilization ( IF 7.7 ) Pub Date : 2020-02-12 , DOI: 10.1016/j.jcou.2020.02.003
Yanxia Zhu , Jun Cheng , Ze Zhang , Jianzhong Liu

In order to promote phenol tolerance and the carbon fixation rate of cells during CO2 sequestration in coal chemical flue gas, γ-ray irradiation from cobalt-60 (60Co) was used to mutate Arthrospira platensis to generate efficient strains. A phenol-tolerant strain Mutant 11k was obtained with 11,000 Gy of gamma irradiation. The phenol degradation efficiency of the mutant was 34.7 % higher than that of wild strain, attributing to the increased activity of phenol hydroxylase that catalyzed phenol to generate catechol. The catechol 1,2-dioxygenase activity in mutant cells was enhanced by 39.5 % while catechol 2,3-dioxygenase was weakened by 23.8 %, revealing that the β-ketoadipate pathway plays a more important role than 2-hydroxymuconic semialdehyde pathway in phenol degradation. The activity of superoxide dismutase and catalase increased by 6.1 % and 39.8 %, respectively, reducing peroxide damage and maintaining rapid cell growth. The contents of chlorophyll-a and maximum quantum yield of photosystem II in mutant cells were higher than those in wild strain. The biomass yield and carbon fixation rate of mutant were 20.8 % and 22.4 % higher, respectively, than those of the wild strain.



中文翻译:

γ辐照突变的节肢螺旋藻以提高苯酚耐受性并固定CO 2以减少煤化学烟气

为了提高煤化学烟道气中CO 2螯合过程中苯酚的耐受性和细胞的碳固定率,使用了钴60(60 Co)的γ射线辐照突变了Arthrospira platensis产生有效的应变。在11,000 Gy的γ射线照射下获得了耐苯酚菌株Mantant 11k。该突变体的苯酚降解效率比野生菌株高34.7%,这归因于催化酚产生儿茶酚的酚羟化酶活性增加。突变细胞中的儿茶酚1,2-二加氧酶活性提高了39.5%,而儿茶酚2,3-二加氧酶的活性降低了23.8%,这表明在酚降解中,β-酮己二酸途径比2-羟基粘康半醛途径更重要。 。超氧化物歧化酶和过氧化氢酶的活性分别增加了6.1%和39.8%,从而减少了过氧化物的破坏并保持了细胞的快速生长。突变细胞中叶绿素a的含量和光系统II的最大量子产率均高于野生菌株。突变体的生物量产量和固碳率分别比野生菌株高20.8%和22.4%。

更新日期:2020-03-05
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