Anxiety disorders (ADs) are nervous system maladies involving changes in the amygdala synaptic circuitry, such as an upregulation of excitatory neurotransmission at glutamatergic synapses. In the field of nanotechnology, thin graphene oxide flakes with nanoscale lateral size (s-GO) have shown outstanding promise for the manipulation of excitatory neuronal transmission with high temporal and spatial precision, thus they were considered as ideal candidates for modulating amygdalar glutamatergic transmission. Here, we validated an in vitro model of amygdala circuitry as a screening tool to target synapses, towards development of future ADs treatments. After one week in vitro, dissociated amygdalar neurons reconnected forming functional networks, whose development recapitulated that of the tissue of origin. When acutely applied to these cultures, s-GO flakes induced a selective modification of excitatory activity. This type of interaction between s-GO and amygdalar neurons may form the basis for the exploitation of alternative approaches in the treatment of ADs.

中文翻译：

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薄的氧化石墨烯纳米薄片调节杏仁核培养回路中的谷氨酸能突触：利用突触方法治疗焦虑症。

焦虑症（ADs）是神经系统疾病，涉及杏仁核突触回路的变化，例如谷氨酸能突触中兴奋性神经传递的上调。在纳米技术领域，具有纳米级横向尺寸（s-GO）的氧化石墨烯薄片显示出以高的时空精度操纵兴奋性神经元传递的杰出前景，因此被认为是调节杏仁核谷氨酸能传递的理想候选者。在这里，我们验证了杏仁核电路的体外模型作为靶向突触的筛选工具，以开发未来的ADs治疗药物。体外培养一周后，解离的杏仁核神经元重新连接，形成功能网络，其发育概括了原始组织的发育。当敏锐地应用于这些文化时，s-GO薄片诱导了兴奋活性的选择性修饰。s-GO和杏仁核神经元之间的这种相互作用类型可能会成为开发替代疗法来治疗AD的基础。