Plant growth promoting bacteria (PGPB) have been used to enhance crop productivity. The effect of native PGPB and arbuscular mycorrhizal (AM) fungi in combination on wheat yield, biofortification and soil enzymatic activity is a relatively unexplored area. Twenty seven bacterial isolates from three different soils were characterized for their plant growth promoting traits. A total of three native and five non-native bacteria were used with and without arbuscular mycorrhizal (AM) fungi in an open greenhouse pot experiment with two wheat varieties to evaluate their effect on wheat yield, nutrient uptake, and soil health parameters. Wheat plants subjected to native PGPB (CP4) (Bacillus subtilis) and AM fungi treatment gave the best results with reference to macronutrient (nitrogen and phosphorus), micronutrient (iron and zinc) content in wheat grains and yield-related parameters, including thousand grain weight, number of grains per spike and total tillers per plant in both wheat cultivars. Treatment with CP4 and CP4 plus AM fungi enhanced total chlorophyll in wheat leaves indicating higher photosynthetic activity. Significant improvement in soil health-related parameters, including soil organic matter and dehydrogenase activity, was observed. Significant correlation among grain yield-related parameters, nutrient enhancement, and soil health parameters was observed in PGPB and AM fungi treated plants, especially HD-3086. These results provide a roadmap for utilizing native PGPB and AM fungi for enhancing wheat production in Punjab state of India and exploring their utility in other parts of the country with different soil and environmental conditions.

植物生长促进细菌（PGPB）已用于提高农作物的生产力。天然PGPB和丛枝菌根（AM）真菌的组合对小麦产量，生物强化作用和土壤酶活性的影响是一个相对未开发的领域。从三种不同土壤中分离出的27种细菌分离株具有促进植物生长的特性。在带有两个小麦品种的开放式温室盆栽试验中，总共使用了三种天然细菌和五种非天然细菌，分别使用和不使用丛枝菌根（AM）真菌，以评估它们对小麦产量，养分吸收和土壤健康参数的影响。小麦植物受天然PGPB（CP4）（枯草芽孢杆菌））和AM真菌处理在小麦籽粒中的大量营养素（氮和磷），微量营养素（铁和锌）含量以及与产量相关的参数（包括千粒重，每个穗粒数和每株总分ers数）方面均达到了最佳效果在两个小麦品种中。用CP4和CP4加AM真菌处理可增强小麦叶片中的总叶绿素，表明较高的光合活性。观察到土壤健康相关参数的显着改善，包括土壤有机质和脱氢酶活性。在PGPB和AM真菌处理过的植物中，尤其是HD-3086，观察到谷物产量相关参数，养分增强和土壤健康参数之间的显着相关。