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SERS-Based Molecularly Imprinted Plasmonic Sensor for Highly Sensitive PAH Detection.
ACS Sensors ( IF 8.9 ) Pub Date : 2020-03-05 , DOI: 10.1021/acssensors.9b01882
Alexander Castro-Grijalba 1, 2 , Verónica Montes-García 1 , María José Cordero-Ferradás 1 , Eduardo Coronado 2 , Jorge Pérez-Juste 1 , Isabel Pastoriza-Santos 1
Affiliation  

A novel hybrid plasmonic platform based on the synergetic combination of a molecularly imprinted polymer (MIP) thin film with Au nanoparticle (NPs) assemblies, noted as Au@MIP, was developed for surface-enhanced Raman scattering (SERS) spectroscopy recognition of polycyclic aromatic hydrocarbons (PAHs). While the MIP trapped the PAH close to the Au surface, the plasmonic NPs enhanced the molecule’s Raman signal. The Au@MIP fabrication comprises a two-step procedure, first, the layer-by-layer deposition of Au NPs on glass and their further coating with a uniform MIP thin film. Profilometry analysis demonstrated that the thickness and homogeneity of the MIP film could be finely tailored by tuning different parameters such as prepolymerization time or spin-coating rate. Two different PAH molecules, pyrene or fluoranthene, were used as templates for the fabrication of pyrene- or fluoranthene-based Au@MIP substrates. The use of pyrene or fluoranthene, as the template molecule to fabricate the Au@MIP thin films, enabled its ultradetection in the nM regime with a 100-fold improvement compared with the nonimprinted plasmonic sensors (Au@NIPs). The SERS data analysis allowed to estimate the binding constant of the template molecule to the MIP. The selectivity of both pyrene- and fluoranthene-based Au@MIPs was analyzed against three PAHs of different sizes. The results displayed the important role of the template molecule used for the Au@MIPs fabrication in the selectivity of the system. Finally, the practical applicability of pyrene-based Au@MIPs was shown by performing the detection of pyrene in two real samples: creek water and seawater. The design and optimization of this type of plasmonic platform will pave the way for the detection of other relevant (bio)molecules in a broad range of fields such as environmental control, food safety, or biomedicine.

中文翻译:

基于SERS的分子印迹等离子传感器,用于高灵敏度PAH检测。

基于分子印迹聚合物(MIP)薄膜与金纳米粒子(NPs)组件的协同组合的新型混合等离子体激元平台,被称为Au @ MIP,用于表面增强拉曼散射(SERS)光谱识别多环芳烃碳氢化合物(PAHs)。当MIP将PAH捕获在Au表面附近时,等离子体NPs增强了分子的拉曼信号。Au @ MIP的制造包括两个步骤,首先是在玻璃上逐层沉积Au NP,然后用均匀的MIP薄膜对其进行进一步涂覆。轮廓分析表明,可以通过调节不同的参数(例如预聚合时间或旋涂速率)来精确调整MIP膜的厚度和均匀性。两种不同的PAH分子,or或荧蒽,用作制备基于pyr或荧蒽的Au @ MIP基片的模板。使用of或荧蒽作为模板分子来制造Au @ MIP薄膜,与非压印等离激元传感器(Au @ NIPs)相比,其在nM机制下的超检测性能提高了100倍。通过SERS数据分析,可以估算模板分子与MIP的结合常数。针对三种不同大小的PAH,分析了基于pyr和荧蒽的Au @ MIPs的选择性。结果显示了用于Au @ MIPs制备的模板分子在系统选择性中的重要作用。最后,通过在两个真实样品(溪水和海水)中进行the的检测,证明了based基Au @ MIPs的实际适用性。
更新日期:2020-03-05
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