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New corrosion cast media and its ability for SEM and light microscope investigation.
Microscopy Research and Technique ( IF 2.5 ) Pub Date : 2020-03-04 , DOI: 10.1002/jemt.23468
Keti Tsomaia 1 , Leila Patarashvili 1 , Irakli Bebiashvili 1 , Elza Azmaiparashvili 1 , Manana Kakabadze 1 , Nikoloz Jalabadze 2 , Marom Sareli 3 , Sergey Gusev 4 , Dimitri Kordzaia 1
Affiliation  

SEM of corrosion casts (CC) provides the opportunities to study the vessels and ducts in the phyllogenetic and ontogenetic (age‐related) settings, as well as the pathogenesis, compensation, and sanogenesis in different diseases and experimental models. Along with the refinement of SEM CC, the requirements toward casting media (CM) as nontoxicity, low viscosity, quick polymerization, resistance to corrosion solutions, availability, and so on, gradually has developed. We aimed to adapt the sets widely used in dental practice toward the modern requirements to the CC. The following ratio of the components of Protacryl‐M and Aycryl‐C sets were used for the preparation CM—0.25 g MAYCRYL Powder +0.08 g Benzoyl Peroxide +5.0 ml Protacryl‐M liquid component +0.2 Redont Colour (dye concentrate). The obtained solidifying mass was injected in the blood vessels and biliary ducts of the adult Wistar white rats. The SEM of CC of different organs’ vascular networks, as well as a biliary tract, reveals that offered CM excellently replicates the forms and branching features of studied tubular structures of all sizes and gives the adequate imprinting of their luminal surfaces. Besides, CM may provide the replication of perivascular spaces and give the casts having no analogous in the appropriate literature. The CM prepared by us perfectly reproduces all possibilities of famous rubbers widely used for the casting of different vascular–ductular structures. Besides, it presents the new implications, which should be implemented in the profound research of the connective‐tissue skeleton of different organs.

中文翻译:

新型腐蚀铸造介质及其用于SEM和光学显微镜研究的能力。

腐蚀铸件(CC)的SEM提供了研究系统发育和个体发育(年龄相关)环境中的血管和导管,以及不同疾病和实验模型中的发病机理,补偿和造血的机会。随着SEM CC的完善,对浇铸介质(CM)的要求逐渐提高,如无毒,低粘度,快速聚合,耐腐蚀溶液,可利用性等。我们旨在使牙科实践中广泛使用的套件适应CC的现代要求。按照以下比例制备Protacryl-M和Aycryl-C组的成分:0.25 g MAYCRYL粉末+0.08 g过氧化苯甲酰+5.0 ml Protacryl-M液体组分+0.2 Redont色(染料浓缩物)。将获得的凝固物注射到成年Wistar白色大鼠的血管和胆管中。不同器官的血管网络以及胆道的CC的SEM图像显示,所提供的CM能够出色地复制各种尺寸的研究管状结构的形式和分支特征,并充分显示其腔表面。此外,CM可以提供血管周围空间的复制,并且在适当的文献中不能提供类似的模型。我们准备的CM完美地再现了广泛用于铸造不同血管-管状结构的著名橡胶的所有可能性。此外,它还提出了新的含义,应在对不同器官的结缔组织骨架进行深入研究时加以实施。
更新日期:2020-03-04
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