Erucic acid (C22:1Δ13) has several industrial applications including its use as a lubricant, surfactant and biodiesel and composite material constituent. It is produced by plants belonging to the Brassicaceae family, especially by the high erucic acid rapeseed. The ability to convert oleic acid into erucic acid is facilitated by FAE1. In this study, FAD2 (encoding Δ12-desaturase) was deleted in the strain Po1d to increase oleic acid content. Subsequently, FAE1 from Thlaspi arvense was overexpressed in Yarrowia lipolytica with the Δfad2 genotype. This resulted in the YL10 strain producing very long chain fatty acids, especially erucic acid. The YL10 strain was cultivated in media containing crude glycerol and waste cooking oil as carbon substrates. The cells grown using glycerol produced microbial oil devoid of linoleic acid, which was enriched with very long chain fatty acids, mainly erucic acid (9% of the total fatty acids). When cells were grown using waste cooking oil, the highest yield of erucic acid was obtained (887 mg L-1). However, external linoleic and α-linolenic were accumulated in cellular lipids when yeasts were grown in an oil medium. This study describes the possibility of conversion of waste material into erucic acid by a recombinant yeast strain.

中文翻译：

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重组酵母解脂耶氏酵母将废物转化成很长的脂肪酸。

芥酸（C22：1Δ13）具有多种工业应用，包括用作润滑剂，表面活性剂，生物柴油和复合材料成分。它是由十字花科的植物生产的，尤其是高芥酸油菜籽。FAE1促进了将油酸转化为芥酸的能力。在这项研究中，删除了菌株Po1d中的FAD2（编码Δ12-去饱和酶）以增加油酸含量。随后，来自Thlaspi arvense的FAE1在具有Δfad2基因型的解脂耶氏酵母中过表达。这导致YL10菌株产生非常长的脂肪酸，尤其是芥酸。将YL10菌株培养在含有粗甘油和​​废食用油作为碳底物的培养基中。使用甘油生长的细胞可产生不含亚油酸的微生物油，富含非常长链的脂肪酸，主要是芥酸（占总脂肪酸的9％）。当使用废食用油培养细胞时，获得了最高的芥酸产率（887 mg L-1）。然而，当酵母在油性培养基中生长时，外部亚油酸和α-亚麻酸会积聚在细胞脂质中。这项研究描述了通过重组酵母菌株将废料转化为芥酸的可能性。