Phytophthora ramorum, P. kernoviae, and P. melonis are each species of current regulatory concern in the United States, the United Kingdom, and other areas of the world. Ex-type material are cultures and duplicates of the type that was used to describe each species and that are deposited in additional culture collections. Using these type specimens as references is essential to designing correct molecular identification and diagnostic systems. Here, we report a whole genome sequence for the Ex-type material of P. ramorum, P. kernoviae, and P. melonis generated using high-throughput sequencing via the MinION third generation platform from Oxford Nanopore Technology. We assembled the quality filtered reads into contigs for each species. We assembled the continuous contigs of P. ramorum, P. kernoviae, and P. melonis (1,322, 545, and 2,091 contigs, respectively). The ab initio prediction of genes from these species reveals that there are 16,838, 12,793, and 34,580 genes in P. ramorum, P. kernoviae, and P. melonis, respectively. Of the 34,580 P. melonis genes, 10,164 genes were conserved among all three of these Phytophthora species which may include pathogenicity genes. We compared the ex-type of P. ramorum EU1 lineage assembly with another selected isolate of EU1 available at the National Center for Biotechnology Information and found 251,859 single nucleotide polymorphisms (SNPs) genome-wide; the comparison with the EU2 lineage genome isolate revealed 441,859 SNPs genome-wide. This genome resource of the ex-types of P. ramorum, and P. kernoviae is a significant contribution as these species are among the most important pathogens of regulatory concern in different regions of the world.

中文翻译：

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使用超长阅读MinION纳米孔测序技术对有关疫霉菌，角腐病菌和瓜病菌的Ex型基因组资源草案进行了研究。

在美国，英国和世界其他地区，疫霉菌，kernoviae和P. melonis都是当前监管关注的物种。防爆型材料是用于描述每种物种的培养物和复制品，它们存放在其他培养物中。使用这些类型的标本作为参考对于设计正确的分子鉴定和诊断系统至关重要。在这里，我们报告了通过牛津纳米孔技术公司的MinION第三代平台使用高通量测序生成的P. ramorum，P。kernoviae和P. melonis Ex型材料的完整基因组序列。我们将每个物种的质量经过筛选的读段组装成重叠群。我们组装了P. ramorum，P。kernoviae和P. melonis的连续重叠群（1,322、545和2,091个重叠群，分别）。从这些物种的基因的从头算的预测表明，在拉美假单胞菌，角鲨假单胞菌和甜瓜假单胞菌中分别有16,838、12,793和34,580个基因。在34,580个瓜果疫霉菌基因中，这三种疫霉属物种中有10,164个基因被保守，其中可能包括致病性基因。我们将拉美假单胞菌EU1谱系装配的前类型与美国国家生物技术信息中心提供的EU1的另一个选定分离株进行了比较，发现全基因组范围内有251,859个单核苷酸多态性（SNP）。与EU2谱系基因组分离物的比较显示全基因组441,859个SNP。ramorum和kernoviae的ex-types的这种基因组资源是重要的贡献，因为这些物种是世界不同地区最重要的监管关注病原体之一。