The zebrafish (Danio rerio) is an ideal model for whole animal studies of lipid metabolism and lipid-related disease. In this work, infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) was applied for direct visualization of lipid and metabolite distributions across various organs in whole-body zebrafish tissue sections. Detailed methods for overcoming the challenges of cryosectioning adult male zebrafish for MSI and complementary histological imaging are described. Representative two-dimensional ion maps demonstrated organ specific localization of lipid analytes allowing for visualization of areas of interest including the brain, liver, intestines, and skeletal muscle. A high resolving power mass spectrometer was utilized for accurate mass measurements, which permitted the use of open-source, web-based tools for MS1 annotations including METASPACE and METLIN. Whole-body MSI with IR-MALDESI allowed for broad lipid coverage with high spatial resolution, illustrating the potential of this technique for studying lipid-related diseases using zebrafish as a model organism.

中文翻译：

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全身斑马鱼冷冻切片和质谱成像的方法。

斑马鱼 (Danio rerio) 是脂质代谢和脂质相关疾病的整个动物研究的理想模型。在这项工作中，红外基质辅助激光解吸电喷雾电离 (IR-MALDESI) 质谱成像 (MSI) 用于直接可视化整个斑马鱼组织切片中各个器官的脂质和代谢物分布。描述了克服对 MSI 和补充组织学成像进行冷冻切片成年雄性斑马鱼挑战的详细方法。具有代表性的二维离子图展示了脂质分析物的器官特异性定位，允许对感兴趣的区域进行可视化，包括大脑、肝脏、肠道和骨骼肌。高分辨率质谱仪用于精确的质量测量，它允许使用开源的、基于 Web 的 MS1 注释工具，包括 METASPACE 和 METLIN。具有 IR-MALDESI 的全身 MSI 允许以高空间分辨率进行广泛的脂质覆盖，说明该技术在使用斑马鱼作为模型生物研究脂质相关疾病方面的潜力。