Recently, attention has shifted to the use of mixed lignocellulosic substrates for the production of cellulolytic enzymes. However, researchers have focused mainly on achieving increased enzyme yields while neglecting other properties of the enzymes when using such mixtures. In this first-ever report of the application of Prosopis africana pod (PAP) in cellulase production, we investigated the effect of its combination with corn cob (CC), as an inducing carbon source, on the amounts and quality of crude endoglucanase produced by Bacillus thuringiensis SS12. The organism was grown on PAP, CC or their 1:1% w/w mixture (MS) and the crude endoglucanases produced were tested for activity, hydrolytic efficiency, and thermostability. PAP supported the highest enzyme activity (0.138 U/mL) and its endoglucanase was the most effective in hydrolyzing CMC and filter paper while CC-derived endoglucanase was the best for hydrolysis of alkali-pretreated CC. Enzyme activity of MS-derived endoglucanase (0.110 U/mL) was intermediate to that of PAP and CC (0.091 U/mL) and was the most stable at elevated temperatures (70 and 80 °C). It also liberated the least amount of reducing sugars from all tested substrates. Combination of both the substrates, thus, favored enzyme production and thermostability but was detrimental to hydrolytic efficiency.

近来，注意力已经转移到使用混合的木质纤维素底物来生产纤维素分解酶。但是，研究人员主要集中在提高酶的产量上，而在使用这种混合物时却忽略了酶的其他特性。在非洲象鼻豆荚（PAP）在纤维素酶生产中的首次应用报告中，我们研究了将其与玉米芯（CC）组合作为诱导碳源，对由纤维素酶生产的粗制内切葡聚糖酶的数量和质量的影响。苏云金芽孢杆菌SS12。该生物在PAP，CC或它们的1：1％w / w混合物（MS）上生长，并测试了产生的粗制内切葡聚糖酶的活性，水解效率和热稳定性。PAP支持最高的酶活性（0.138 U / mL），其内切葡聚糖酶对水解CMC和滤纸最有效，而CC衍生的内切葡聚糖酶对碱预处理的CC水解效果最好。MS衍生的内切葡聚糖酶（0.110 U / mL）的酶活性介于PAP和CC（0.091 U / mL）的中间，并且在升高的温度（70和80°C）下最稳定。它还从所有测试的底物中释放出最少量的还原糖。因此，两种底物的组合有利于酶的产生和热稳定性，但是不利于水解效率。