AIM OF STUDY This investigation evaluated the capacity of epigallocatechin-3-gallate (EGCG) as the main polyphenolic compound in the green tea extract against memory impairment and neurotoxicity in morphine-treated rats. METHODS To measure the EGCG effect (5 and 50 mg/kg, i.p., co-treated with morphine) on spatial learning and memory of morphine-administrated male Wistar rats (45 mg/kg, s.c., 4 weeks), the Morris water maze test was used. Some apoptotic protein levels (Bax, Bcl-2, and cleaved caspase 3) were evaluated in the hippocampus tissue by the Western blot test. Also, oxidative stress status (malondialdehyde level, glutathione peroxidase, and superoxide dismutase activity) was measured in hippocampus tissue. RESULTS The data presented that EGCG treatment (50 mg/kg) inhibited the morphine-induced memory deficits in rats. Also, EGCG administration reduced the apoptosis and oxidative stress in the hippocampus of morphine-treated rats. CONCLUSIONS Our data indicate that EGCG can improve memory in morphine-treated rats. Molecular mechanisms underlying the detected effects could be related to the prevention of apoptosis and oxidative stress in the hippocampus of morphine-treated rats.

中文翻译：

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表没食子儿茶素-3-没食子酸酯对吗啡引起的大鼠记忆障碍的影响：EGCG 对吗啡神经毒性的影响。

研究目的 本研究评估了作为绿茶提取物中主要多酚化合物的表没食子儿茶素-3-没食子酸酯 (EGCG) 对吗啡治疗大鼠的记忆障碍和神经毒性的能力。方法 测量 EGCG 对吗啡给药雄性 Wistar 大鼠（45 mg/kg，sc，4 周）、Morris 水迷宫空间学习和记忆的影响（5 和 50 mg/kg，ip，与吗啡共同治疗）。使用了测试。通过蛋白质印迹试验评估海马组织中的一些凋亡蛋白水平（Bax、Bcl-2 和切割的半胱天冬酶 3）。此外，还测量了海马组织中的氧化应激状态（丙二醛水平、谷胱甘肽过氧化物酶和超氧化物歧化酶活性）。结果 数据表明，EGCG 治疗（50 mg/kg）可抑制吗啡引起的大鼠记忆缺陷。还，EGCG 给药减少了吗啡处理的大鼠海马中的细胞凋亡和氧化应激。结论 我们的数据表明，EGCG 可以改善吗啡治疗大鼠的记忆力。检测到的作用背后的分子机制可能与预防吗啡处理的大鼠海马中的细胞凋亡和氧化应激有关。