An athlete challenged the result from an in-competition doping test which returned with an adverse analytical finding for stanozolol, claiming it was due to supplement contamination. Her lawyer asked the laboratory to analyze several hair specimens simultaneously collected from five different anatomical regions, head, arm, leg, pubis and armpit, to document the pattern of drug exposure. A specific UPLC–MS-MS method was developed. After decontamination with dichloromethane, stanozolol was extracted from hair in the presence of stanozolol-d₃ used as internal standard, under alkaline conditions, with diethyl ether. Linearity was observed for concentrations ranging from 5 pg/mg to 10 ng/mg. The method has been validated according to linearity, precision and matrix effect. Concentrations of stanozolol in head hair, pubic hair, arm hair, leg hair and axillary hair were 73, 454, 238, 244 and 7,100 pg/mg, respectively. The concentration of stanozolol in head hair is in accordance with data published in the literature. When comparing the concentrations, body hair concentrations were higher than the concentration found in head hair. These results are consistent with a better incorporation rate of stanozolol in body hair when compared to head hair. The simultaneous positive concentrations in different hair types confirm the adverse analytical finding in urine of the top athlete, as the measured concentrations do not support the theory of contamination. For the first time, an anabolic agent was simultaneously tested in hair collected from five different anatomical regions from the same subject, with a large distribution of concentrations, due to anatomical variations, and these findings will help interpretation in further doping cases when documented with hair.

中文翻译：

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使用UPLC-MS / MS并通过UPLC-q-TOF-MS确认从5个不同的解剖区域收集的头发样本中的替诺洛尔含量。

一名运动员对一项竞赛中的兴奋剂测试结果提出了质疑，该测试结果归因于替诺洛尔的不利分析发现，称它是由于补充污染物所致。她的律师要求实验室分析同时从五个不同的解剖区域（头部，手臂，腿部，耻骨和腋窝）收集的几个头发样本，以记录药物暴露的方式。开发了一种特定的UPLC-MS-MS方法。用二氯甲烷净化后，在存在stanozolol-d ₃的情况下从头发中提取stanozolol在碱性条件下，与乙醚一起用作内标。浓度在5 pg / mg至10 ng / mg范围内观察到线性。该方法已根据线性，精度和矩阵效应进行了验证。头唑，阴毛，臂毛，腿毛和腋毛中司坦唑醇的浓度分别为73、454、238、244和7100 pg / mg。头发中司坦洛尔的浓度与文献中公布的数据一致。比较浓度时，体毛浓度高于头发中的浓度。与头发相比，这些结果与替诺洛尔在头发中的掺入率更高相符。不同头发类型中同时出现的正浓度证实了顶级运动员尿液中不利的分析发现，因为测得的浓度不支持污染理论。第一次，从同一受试者的五个不同解剖区域收集的头发中同时测试了合成代谢药物，由于解剖学差异，其浓度分布较大，这些发现将有助于在进一步记录掺杂头发的情况下进行解释。