The protected niche of deep-caries lesions is a distinctive ecosystem. We assessed the Candida biome and its cariogenic traits from dentin samples of 50 children with severe-early childhood caries (S-ECC). Asymptomatic, primary molars belonging to International Caries Detection and Assessment-ICDAS caries-code 5 and 6 were analyzed, and C. albicans (10-isolates), C. tropicalis (10), C. krusei (10), and C. glabrata (5) isolated from the lesions were then evaluated for their biofilm formation, acidogenicity, and the production of secreted hydrolases: hemolysins, phospholipase, proteinase and DNase. Candida were isolated from 14/43 ICDAS-5 lesions (32.5%) and 44/57 ICDAS-6 lesions (77.2%). Compared to, ICDAS-5, a significantly higher frequency of multi-species infestation was observed in ICDAS-6 lesions (p=0.001). All four candidal species (above) showed prolific biofilm growth, and an equal potency for tooth demineralization. A significant interspecies difference in the mean phospholipase, as well as proteinase activity was noted (p < 0.05), with C. albicans being the predominant hydrolase producer. Further, a positive correlation between phospholipase and proteinase activity of Candida-isolates was noted (r = 0.818, p < 0.001). Our data suggest that candidal mycobiota with their potent cariogenic traits may significantly contribute to the development and progression of S-ECC.

中文翻译：

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严重幼儿龋齿（S-ECC）的念珠菌生物群及其致龋性毒力特征。

深龋病灶的受保护生态位是一个独特的生态系统。我们从50名患有严重幼儿期龋齿（S-ECC）的儿童的牙本质样品中评估了念珠菌的生物群落及其致龋性状。分析了无症状的，属于国际龋齿检测和评估-ICDAS龋齿代码5和6的主要磨牙，并检测了白色念珠菌（10个分离株），热带念珠菌（10），克鲁氏梭菌（10）和glabrata （5）然后评估从病变中分离出的生物膜的形成，产酸性以及分泌的水解酶的产生：溶血素，磷脂酶，蛋白酶和DNase。从14/43 ICDAS-5病变（32.5％）和44/57 ICDAS-6病变（77.2％）中分离出假丝酵母。与ICDAS-5相比，在ICDAS-6病变中观察到多物种感染的频率明显更高（p = 0.001）。所有四个候选物种（以上）均显示出丰富的生物膜生长，并且牙齿脱矿质的能力相同。注意到平均磷脂酶以及蛋白酶活性之间存在明显的种间差异（p <0.05），白色念珠菌是主要的水解酶生产商。此外，注意到念珠菌分离物的磷脂酶和蛋白酶活性之间呈正相关（r = 0.818，p <0.001）。我们的数据表明，念珠菌分枝杆菌具有强大的致龋性，可能显着促进S-ECC的发展和进程。念珠菌分离物的磷脂酶和蛋白酶活性之间呈正相关（r = 0.818，p <0.001）。我们的数据表明，念珠菌分枝杆菌具有强大的致龋性，可能显着促进S-ECC的发展和进程。念珠菌分离物的磷脂酶和蛋白酶活性之间呈正相关（r = 0.818，p <0.001）。我们的数据表明，念珠菌分枝杆菌具有强大的致龋性，可能显着促进S-ECC的发展和进程。