Intact protein mass spectrometry (MS) via electrospray-based methods is often degraded by low-mass contaminants, which can suppress the spectral quality of the analyte of interest via space-charge effects. Consequently, selective removal of contaminants by their mobilities would benefit native MS if achieved without additional hardware and before the mass analyzer regions used for selection, analyte readout, or tandem MS. Here, we use the high-pressure multipole within the source of an Orbitrap Tribrid as the foundation for a coarse ion filter. Using this method, we show complete filtration of 2 mM polyethylene glycol (PEG-1000) during native MS of SILu mAb antibody present at a 200× lower concentration. We also show the generality of the process by rescuing 10 μM tetrameric pyruvate kinase from 2 mM PEG-1000, asserting this voltage rollercoaster filtering (VRF) method for use in native MS as an efficient alternative to conventional purification methods.

中文翻译：

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天然蛋白质分析过程中低质量污染物的电压过山车过滤。

通过基于电喷雾的方法的完整蛋白质质谱（MS）通常会因低质量污染物而降解，这会通过空间电荷效应抑制目标分析物的光谱质量。因此，如果在没有附加硬件的情况下并且在用于选择，分析物读出或串联质谱的质量分析器区域之前实现，通过污染物的迁移性选择性去除污染物将有利于天然质谱。在这里，我们使用Orbitrap Tribrid源中的高压多极作为粗离子过滤器的基础。使用此方法，我们显示出以200x较低浓度存在的SILu mAb抗体的天然MS中2 mM聚乙二醇（PEG-1000）的完全过滤。我们还通过从2 mM PEG-1000中拯救10μM四聚丙酮酸激酶来显示该过程的一般性，