AIMS We investigated whether a lifestyle intervention could influence expression and DNA methylation of diabetes-related genes in patients with impaired glucose regulation (IGR), the results were compared to bariatric surgery, considering it an intensive change. METHODS Twenty participants with IGR had adipose tissue biopsy and blood collected pre- and post-lifestyle (6 months) intervention; 12 obese patients had subcutaneous fat taken before and after bariatric surgery. RNA/DNA was extracted from all samples and underwent qPCR. DNA was bisulphite converted and 12 CpG sites of Caveolin-1 (CAV1) promoter were pyrosequenced. RESULTS lifestyle intervention resulted in opposite direction changes in fat tissue and blood for CAV1 expression and DNA methylation and these changes were correlated between tissues, while no significative differences were found in CAV1 expression after bariatric surgery. CONCLUSIONS Our findings suggest a role for CAV1 in modulating adipocyte function as a consequence of lifestyle changes, as exercises and diet. These results may provide insights into new therapeutic targets for diabetes prevention.

中文翻译：

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葡萄糖调节受损的个体的生活方式干预会影响Caveolin-1的表达和DNA甲基化。

目的我们研究了生活方式干预是否会影响葡萄糖调节受损（IGR）患者的糖尿病相关基因的表达和DNA甲基化，将结果与减肥手术进行了比较，认为这是一个剧烈的变化。方法20名IGR参与者在进行生活方式前和后（6个月）干预后进行了脂肪组织活检并采集了血液。肥胖患者在减肥手术前后均采取皮下脂肪治疗，其中12例肥胖。从所有样品中提取RNA / DNA并进行qPCR。DNA被亚硫酸氢盐转化，并且对Caveolin-1（CAV1）启动子的12个CpG位点进行了焦磷酸测序。结果生活方式干预导致脂肪组织和血液中CAV1表达和DNA甲基化的方向相反，这些变化与组织之间相关，减肥手术后，CAV1表达无明显差异。结论我们的研究结果表明，由于运动和饮食方式的改变，CAV1在调节脂肪细胞功能中的作用。这些结果可以为预防糖尿病的新治疗靶标提供见识。