The entomopathogenic fungi Metarhizium anisopliae is highly pathogenic toward arthropods. Here, we evaluated the efficacy of a commercial formulation of M. anisopliae against P. ovis var. cuniculi in vivo and in vitro and explored the acaricidal mechanism of M. anisopliae by determining the antioxidant/detoxification-related enzymes activities including glutathione S-transferase (GST), superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in mites. The results showed that M. anisopliae had high acaricidal activity against P. ovis var. cuniculi in vitro, in a time- and dose-dependent manner, with 83.33 % mortality at day 9 and a median lethal time (LT50) of 6.10 days after applying 6.14 × 109 conidia/ml of M. anisopliae. In vivo experiments, M. anisopliae achieved 100 % therapeutic effect after 3 days, compared with only 62.21 % for ivermectin. Enzyme assays showed that M. anisopliae significantly upregulated activities of GST, SOD and CAT in Psoroptes mites. The results indicate that M. anisopliae may be an effective biological agent for control of P. ovis var. cuniculi infestations in rabbits and the acaricidal activity may be associated with the changes of enzyme activities of the detoxification and antioxidant system in Psoroptes mites.

中文翻译：

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拟南芥变种CQMa128调节抗氧化/解毒酶，并具有对羊伪孢的杀螨活性。兔子的尾uli：初步研究。

昆虫病原真菌Metanhizium anisopliae对节肢动物具有高度致病性。在这里，我们评估了M. anisopliae的商业配方对P. ovis var的功效。体内和体外通过研究谷胱甘肽的抗氧化作用，通过测定抗氧化/解毒相关酶的活性，包括谷胱甘肽S-转移酶（GST），超氧化物歧化酶（SOD），过氧化物酶（POD）和过氧化氢酶（CAT）螨。结果表明，南美分枝杆菌对P. ovis var具有较高的杀螨活性。以6.14×109分生孢子/ ml的M. anisopliae，在第9天的体外致死率和剂量依赖性，在第9天的死亡率为83.33％，中位致死时间（LT50）为6.10天。在体内实验中，南美分枝杆菌在3天后达到100％的治疗效果，而只有62天。伊维菌素为21％。酶法检测结果表明，分离的分枝杆菌明显上调了伪螨的GST，SOD和CAT活性。结果表明，南美分枝杆菌可能是一种有效的生物因子，可以控制羊驼毛。家兔的钩虫感染和杀螨活性可能与褐螨的解毒和抗氧化系统的酶活性变化有关。