Lard is a by-product of animal processing. It is inexpensive compared with vegetable oils; however, its use is limited due to the high calorific value and high-saturated fatty acid content. While using lard as the source of free fatty acids (FFA) can significantly increase its utilization value. This study aimed to research the method on efficient hydrolysis of lard catalyzed by combi-lipases and assisted with ultrasound pretreatment. A 1,3-specific lipase from Rhizomucor miehei (termed pRML, 1540 U/mL) and a nonspecific mono- and diacylglycerol lipase from Penicillium cyclopium (termed MDL, 2000 U/mL) were used as biocatalysts. Results showed that the maximum hydrolysis rate of lard after 6 h at 45°C by using pRML and MDL alone was, respectively, 39.9% and 8.5%. When pRML combined with MDL (combi-lipases), hydrolysis rate can reach to 78.1%. While combi-lipases were assisted with 5 min ultrasound pretreatment before the reaction, the hydrolysis rate can further increase to 97%. The combi-lipases with different specificity and assisted with ultrasound pretreatment may be a useful technology for the enzyme production of FFA from complex lipid substrates, such as lard.

中文翻译：

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由两种脂肪酶在水介质中催化的超声波辅助水解猪油以获得游离脂肪酸。

猪油是动物加工的副产品。与植物油相比价格便宜；但由于其热值高、饱和脂肪酸含量高，其用途受到限制。而使用猪油作为游离脂肪酸（FFA）的来源可以显着提高其利用价值。本研究旨在研究联合脂肪酶催化、超声预处理辅助下高效水解猪油的方法。来自米黑根毛霉的 1,3 特异性脂肪酶（称为 pRML，1540 U/mL）和来自青霉的非特异性单酰基甘油和二酰基甘油脂肪酶（称为 MDL，2000 U/mL）用作生物催化剂。结果表明，单独使用pRML和MDL在45℃下6 h后猪油的最大水解率分别为39.9%和8.5%。当pRML与MDL（组合脂肪酶）结合时，水解率可达78.1%。而组合脂肪酶在反应前辅以5分钟超声波预处理，水解率可进一步提高至97%。具有不同特异性并辅助超声预处理的组合脂肪酶可能​​是从复杂的脂质底物（例如猪油）酶生产 FFA 的有用技术。