Mycobacterial diseases are important health issues in farmed deer. The single intradermal tuberculin test is the standard test for tuberculosis testing in deer. We studied two factors which might influence the response of deer to skin testing: the inoculation site and the injection device. Deer included in this study were 2.5 years old farmed red deer (Cervus elaphus) hinds (n = 80). Two areas of 3 cm × 3 cm were shaved at the left side of the neck. Site A (SA) was situated about 10 cm caudal to the head, while site B (SB) was 10 cm caudal to SA. All hinds received at the same time two 0.1 ml inoculations of Mycobacterium avium derived purified protein derivative (aPPD). One inoculation was made by syringe and the other one with the needle-free syringe Dermojet. To test the inoculation site effect, half of the animals were inoculated by Dermojet in SA and by syringe in SB to compare with the inoculation in SA by syringe and Dermojet in SB in the other half. No differences were recorded for the injection device nor for the inoculation site. Ten hinds had a skinfold increase larger than 30 tenths of mm by any injection device and inoculation site. Seven (9%) and 6 (8%) hinds were classified as positive by syringe and Dermojet, and at the anterior or posterior inoculation site, respectively. The distribution of skinfold thickness increases did not differ by injection device. Our findings support the needle-free Dermojet syringe as a suitable tool for skin-testing in red deer and suggest no relevant effect of the position of the inoculation site along the neck in red deer.

中文翻译：

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接种部位和注射装置对马鹿派生自鸟分枝杆菌的纯化蛋白衍生物（PPD）皮内注射对马鹿的皮肤测试反应没有影响。

分枝杆菌疾病是养殖鹿的重要健康问题。单个皮内结核菌素检测是鹿结核病检测的标准检测。我们研究了可能影响鹿对皮肤测试反应的两个因素：接种部位和注射装置。这项研究中包括的鹿是2.5岁的养殖马鹿（Cervus elaphus）后代（n = 80）。在颈部左侧剃去3厘米×3厘米的两个区域。部位A（SA）位于头部尾部约10厘米处，而部位B（SB）在SA尾部约10厘米处。所有后代同时接受两次0.1 ml接种的鸟分枝杆菌衍生的纯化蛋白衍生物（aPPD）。一种用注射器接种，另一种用无针注射器Dermojet接种。为了测试接种部位的效果，将一半的动物在SA中通过Dermojet接种，在SB中通过注射器接种，与另一半中在SSB中通过注射器和Dermojet接种在SB中进行比较。没有记录注射装置或接种部位的差异。通过任何注射装置和接种部位，十个后肢的皮褶增加都大于30个十分之一毫米。通过注射器和Dermojet分别在前或后接种部位将七（9％）和6（8％）的后代归为阳性。皮褶厚度增加的分布因注射装置而无差异。我们的研究结果支持无针Dermojet注射器作为马鹿皮肤测试的合适工具，并提示马鹿沿颈部的接种部位位置无相关影响。