T almost half a century from the first use of immunohistochemistry (IHC) in routinely processed tissues the method must be counted a success, beyond expectations. IHC is part of Surgical Pathology everywhere; essential in clinical diagnosis, pervading every surgical pathology textbook, intrinsic to much research and a key method in the great majority of published manuscripts in the field. Now is a good time to consider how the foundations of IHC impact the current practice of IHC, contributing to its strengths and its ongoing weaknesses, as an approach to moving forward to the higher levels of performance required for Precision Pathology. Just as the present finds its roots in the past, so also does the future.

中文翻译：

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免疫组织化学和分子形态学的里程碑

在常规处理的组织中首次使用免疫组织化学 (IHC) 近半个世纪以来，该方法必须被视为成功，超出预期。IHC 是无处不在的外科病理学的一部分；在临床诊断中必不可少，遍及每本外科病理学教科书，是许多研究的固有内容，也是该领域绝大多数已发表手稿中的关键方法。现在是考虑 IHC 的基础如何影响 IHC 当前实践的好时机，有助于其优势和持续存在的弱点，作为推进精密病理学所需更高水平性能的方法。正如现在在过去扎根一样，未来也是如此。