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Function of ceramide synthases on growth, ganoderic acid biosynthesis and sphingolipid homeostasis in Ganoderma lucidum
Phytochemistry ( IF 3.8 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.phytochem.2020.112283
Xiaoxiao Lu 1 , Chunqin Xie 2 , Yunxiao Wang 1 , Yongnan Liu 1 , Jing Han 1 , Liang Shi 1 , Jing Zhu 1 , Hanshou Yu 1 , Ang Ren 1 , Mingwen Zhao 1
Affiliation  

Ceramide synthases (CERSs) catalyse an N-acyltransferase reaction using long-chain base (LCB) and fatty acyl-coenzyme A (CoA) as substrates to synthesize ceramide (Cer), which is the backbone of all complex sphingolipids. In the present study, three CERSs (LAG1, LAG2 and LAG3) form Ganoderma lucidum were analysed. The silencing of lag1 by RNA interference reduced ganoderic acid biosynthesis and Cer and complex sphingolipids contents, which contain long-chain-fatty-acids (LCFAs, including C16 and C18). In contrast, the silencing of lag2 or lag3 did not result in obvious phenotypic and sphingolipid homeostasis changes, although the lag2/lag3 double-silenced mutants exhibited increased ganoderic acid biosynthesis as well as reduced growth, reduced Cer and complex sphingolipids contents, which contain very-long-chain fatty acids (VLCFAs, including C22, C24 and C26). The results of the present study indicate that the three assayed CERSs have distinct physiological functions and substrate specificities in G. lucidum.

中文翻译:

神经酰胺合成酶对灵芝生长、灵芝酸生物合成和鞘脂稳态的作用

神经酰胺合酶 (CERS) 使用长链碱基 (LCB) 和脂肪酰基辅酶 A (CoA) 作为底物催化 N-酰基转移酶反应,合成神经酰胺 (Cer),神经酰胺 (Cer) 是所有复杂鞘脂的骨架。在本研究中,分析了灵芝的三种 CERS(LAG1、LAG2 和 LAG3)。RNA干扰对lag1的沉默降低了灵芝酸的生物合成以及Cer和复合鞘脂的含量,其中含有长链脂肪酸(LCFA,包括C16和C18)。相比之下,lag2 或lag3 的沉默并未导致明显的表型和鞘脂稳态变化,尽管lag2/lag3 双沉默突变体表现出灵芝酸生物合成增加以及生长减少、Cer 和复合鞘脂含量降低,其中含有非常- 长链脂肪酸(VLCFA,包括 C22、C24 和 C26)。本研究的结果表明,三种测定的 CERS 在灵芝中具有不同的生理功能和底物特异性。
更新日期:2020-04-01
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