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Vaginal dysbiosis associated-bacteria Megasphaera elsdenii and Prevotella timonensis induce immune activation via dendritic cells.
Journal of reproductive immunology Pub Date : 2020-01-22 , DOI: 10.1016/j.jri.2020.103085
Nienke H van Teijlingen 1 , Leanne C Helgers 1 , Esther M Zijlstra-Willems 1 , John L van Hamme 1 , Carla M S Ribeiro 1 , Karin Strijbis 2 , Teunis B H Geijtenbeek 1
Affiliation  

Dysbiosis of the vaginal microbiome as a result of overgrowth of anaerobic bacteria leads to bacterial vaginosis (BV) which is associated with increased inflammation in the genital mucosa. Moreover, BV increases susceptibility to sexual transmitted infections (STIs) and is associated with adverse pregnancy outcomes. It remains unclear how specific vaginal aerobic and anaerobic bacteria affect health and disease. We selected different vaginal bacteria ranging from true commensals to species associated with dysbiosis and investigated their effects on activation of dendritic cells (DCs). Commensal Lactobacilli crispatus did not induce DC maturation nor led to production of pro-inflammatory cytokines. In contrast, BV-associated bacteria Megasphaera elsdenii and Prevotella timonensis induced DC maturation and increased levels of pro-inflammatory cytokines. Notably, DCs stimulated with Prevotella timonensis suppressed Th2 responses and induced Th1 skewing, typically associated with preterm birth. In contrast, Lactobacillus crispatus and Megasphaera elsdenii did not affect Th cell polarization. These results strongly indicate that the interaction of vaginal bacteria with mucosal DCs determines mucosal inflammation and we have identified the anaerobic bacterium Prevotella timonensis as a strong inducer of inflammatory responses. Specifically targeting these inflammation-inducing bacteria might be a therapeutic strategy to prevent BV and associated risks in STI susceptibility and preterm birth.

中文翻译:

阴道生态失调相关细菌 Megasphaera elsdenii 和 Prevotella timonensis 通过树突状细胞诱导免疫激活。

厌氧菌过度生长导致阴道微生物群失调导致细菌性阴道病 (BV),这与生殖器粘膜炎症增加有关。此外,BV 增加了对性传播感染 (STI) 的易感性,并与不良妊娠结局相关。目前尚不清楚特定的阴道需氧菌和厌氧菌如何影响健康和疾病。我们选择了不同的阴道细菌,从真正的共生菌到与生态失调相关的物种,并研究了它们对树突状细胞 (DC) 激活的影响。共生的卷曲乳杆菌不会诱导 DC 成熟,也不会导致促炎细胞因子的产生。相比之下,BV 相关细菌 Megasphaera elsdenii 和 Prevotella timonensis 诱导 DC 成熟并增加促炎细胞因子水平。值得注意的是,用普雷沃氏菌刺激的 DC 抑制了 Th2 反应并诱导了 Th1 偏斜,这通常与早产有关。相反,Lactobacillus crispatus 和 Megasphaera elsdenii 不影响 Th 细胞极化。这些结果有力地表明,阴道细菌与粘膜 DC 的相互作用决定了粘膜炎症,我们已将厌氧菌普雷沃氏菌鉴定为炎症反应的强诱导剂。专门针对这些炎症诱导细菌可能是预防 BV 和 STI 易感性和早产相关风险的治疗策略。用普雷沃氏菌刺激的 DC 会抑制 Th2 反应并诱导 Th1 偏斜,这通常与早产有关。相反,Lactobacillus crispatus 和 Megasphaera elsdenii 不影响 Th 细胞极化。这些结果有力地表明,阴道细菌与粘膜 DC 的相互作用决定了粘膜炎症,我们已将厌氧菌普雷沃氏菌鉴定为炎症反应的强诱导剂。专门针对这些炎症诱导细菌可能是预防 BV 和 STI 易感性和早产相关风险的治疗策略。用普雷沃氏菌刺激的 DC 会抑制 Th2 反应并诱导 Th1 偏斜,这通常与早产有关。相反,Lactobacillus crispatus 和 Megasphaera elsdenii 不影响 Th 细胞极化。这些结果有力地表明,阴道细菌与粘膜 DC 的相互作用决定了粘膜炎症,我们已将厌氧菌普雷沃氏菌鉴定为炎症反应的强诱导剂。专门针对这些炎症诱导细菌可能是预防 BV 和 STI 易感性和早产相关风险的治疗策略。这些结果有力地表明,阴道细菌与粘膜 DC 的相互作用决定了粘膜炎症,我们已将厌氧菌普雷沃氏菌鉴定为炎症反应的强诱导剂。专门针对这些炎症诱导细菌可能是预防 BV 和 STI 易感性和早产相关风险的治疗策略。这些结果有力地表明,阴道细菌与粘膜 DC 的相互作用决定了粘膜炎症,我们已将厌氧菌普雷沃氏菌鉴定为炎症反应的强诱导剂。专门针对这些炎症诱导细菌可能是预防 BV 和 STI 易感性和早产相关风险的治疗策略。
更新日期:2020-01-22
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