Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018.,Emerging Microbes & Infections

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Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018.
Emerging Microbes & Infections ( IF 13.2 ) Pub Date : 2020-01-29 , DOI: 10.1080/22221751.2020.1717999
Yanqun Wang ₁ , Xin Li ₁ , Wenkuan Liu ₁ , Mian Gan ₁ , Lu Zhang ₂ , Jin Wang ₁ , Zhaoyong Zhang ₁ , Airu Zhu ₁ , Fang Li ₁ , Jing Sun ₁ , Guoxian Zhang ₁ , Zhen Zhuang ₁ , Jiaying Luo ₁ , Dehui Chen ₁ , Shuyan Qiu ₁ , Li Zhang ₁ , Duo Xu ₁ , Chris Ka Pun Mok _{1,

3} , Fuchun Zhang ₂ , Jingxian Zhao ₁ , Rong Zhou ₁ , Jincun Zhao _{1,

2}

Affiliation

Human coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and single amino acid polymorphism analyses showed that this outbreak was associated with two subgenotypes (C3 and B) of HCoV-NL63. Half of patients were identified to be related to a new subgenotype C3. One unique amino acid mutation at I507 L in spike protein receptor binding domain (RBD) was detected, which segregated this subgenotype C3 from other known subgenotypes. Pseudotyped virus bearing the I507 L mutation in RBD showed enhanced entry into host cells as compared to the prototype virus. This study proved that HCoV-NL63 was undergoing continuous mutation and has the potential to cause severe lower respiratory disease in humans.

更新日期：2020-01-29

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