The anaerobic bacterium Clostridioides difficile is the leading cause of antibiotic-associated diarrhea that can culminate in life-threating colitis. During the C. difficile infection (CDI), C. difficile produces toxins that generate the clinical symptoms of the disease, and produce spores, which persist in the host during antibiotic treatment and can cause recurrent CDI (R-CDI). In this work, we aimed to compare three antibiotic regimens in the susceptibility of mice to CDI and R-CDI (i.e., antibiotic cocktail followed by clindamycin, 5 days of cefoperazone and 10 days of cefoperazone) with three different C. difficile isolates (i.e., strains 630; R20291, and VPI 10463). We observed that the severity of the clinical symptoms of CDI and R-CDI was dependent on the antibiotic treatment used to induce C. difficile-susceptibility, and that the three strains generated a different onset to diarrhea and weight loss in mice that were administrated with the same antibiotic treatment and which differed in comparison to the effect previously reported by other research groups. Our results suggest that, in our experimental conditions, in those animals treated with antibiotic cocktail followed by clindamycin, infection with strain R20291 had the highest diarrhea manifestation in comparison to strains 630 and VPI 10463. In animals treated with cefoperazone for 5 days, infection with strains R20291 or 630 had the highest diarrhea manifestation in comparison to VPI 10463, while in animals treated with cefoperazone for 10 days, infection with strain R20291 or VPI 10463, but not 630, had the highest diarrhea manifestation.

厌氧细菌梭状芽胞杆菌是导致与抗生素相关的腹泻的主要原因，这种腹泻最终可导致致命的结肠炎。在艰难梭菌感染（CDI）期间，艰难梭菌产生的毒素会产生该疾病的临床症状，并产生孢子，这些孢子在抗生素治疗期间会持续存在于宿主体内，并可能导致复发性CDI（R-CDI）。在这项工作中，我们旨在比较三种抗生素对小鼠对CDI和R-CDI的易感性（即，抗生素混合物后加克林霉素，头孢哌酮5天和头孢哌酮10天）与三种艰难梭菌的敏感性分离株（即菌株630； R20291和VPI 10463）。我们观察到CDI和R-CDI的临床症状严重程度取决于诱导艰难梭菌的抗生素治疗-易感性，并且三种菌株在使用相同抗生素治疗的小鼠中产生不同的腹泻和体重减轻发作，并且与其他研究小组先前报道的效果相比有所不同。我们的结果表明，在我们的实验条件下，与抗生素菌株630和VPI 10463相比，在用抗生素鸡尾酒接着用克林霉素治疗的动物中，与菌株630和VPI 10463相比，感染R20291的腹泻表现最高。在接受头孢哌酮治疗5天的动物中，感染与VPI 10463相比，R20291或630菌株的腹泻表现最高，而在用头孢哌酮处理10天的动物中，R20291或VPI 10463而不是630的感染具有最高的腹泻表现。