Pyricularia oryzae is the causal agent of blast disease on staple gramineous crops. Sulphur is an essential element for the biosynthesis of cysteine and methionine in fungi. Here, we targeted the P. oryzae PoMET3 encoding the enzyme ATP sulfurylase, and PoMET14 encoding the APS (adenosine-5′-phosphosulphate) kinase that are involved in sulfate assimilation and sulphur-containing amino acids biosynthesis. In P. oryzae, deletion of PoMET3 or PoMET14 separately results in defects of conidiophore formation, significant impairments in conidiation, methionine and cysteine auxotrophy, limited invasive hypha extension, and remarkably reduced virulence on rice and barley. Furthermore, the defects of the null mutants could be restored by supplementing with exogenous cysteine or methionine. Our study explored the biological functions of sulfur assimilation and sulphur-containing amino acids biosynthesis in P. oryzae.

稻瘟病菌（Pyricularia oryzae）是稻瘟病作物上稻瘟病的病因。硫是真菌中半胱氨酸和蛋氨酸生物合成的重要元素。在这里，我们以P为目标。米曲霉PoMET3编码酶ATP硫酸，和PoMET14编码参与硫酸盐同化和含硫氨基酸生物合成中的APS（腺苷-5'-三phosphosulphate）激酶。在P中。米曲霉，缺失PoMET3或PoMET14分别导致of虫形成缺陷，大孢子，蛋氨酸和半胱氨酸营养缺陷重大损害，侵入性菌丝延伸受限，以及大米和大麦的毒力明显降低。此外，可以通过补充外源半胱氨酸或蛋氨酸来修复无效突变体的缺陷。我们的研究探讨硫同化和含硫氨基酸生物合成的生物功能P。菌。