Bisphenol A (BPA), diethylhexyl phthalate (DEHP) and pentabrominated diphenyl ether 99 (PBDE 99) are environmental toxicants belonging to the endocrine disrupting compounds (EDCs). They exert adverse effects on the various physiological systems, especially the reproductive system of humans and animals. The aim of this study was to investigate the effects of BPA, DEHP and PBDE 99 on progesterone (P4) synthesis in cultured bovine luteal cells. The bovine luteal cells isolated from the mid‐luteal corpora lutea were exposed to different concentrations of BPA (1, 3, 10 and 30 µM), DEHP (1, 3, 10 and 30 µM) and PBDE 99 (0.1, 0.3, 1 and 3 µM) in a serum‐free culture media for 48 and 96 hr. At 48 hr, the P4 level in the luteal cells decreased after treatment with all concentrations of BPA; 3, 10 and 30 µM of DEHP; and 3 µM of PBDE 99 compared to the control (p  < .05). Treatment of cells with 3–30 µM of BPA, 1–30 µM of DEHP and 1–3 µM of PBDE 99 for 96 hr resulted in reduction in P4 synthesis (p  < .05). However, lower concentrations of PBDE 99 (0.1 and 0.3 µM) increased P4 levels at 48 and 96 hr. Synthesis of P4 was lower at 96 hr compared to the 48 hr in the groups treated with BPA (30 µM), DEHP (1–30 µM), PBDE 99 (0.3–3 µM) and control group. Our results showed that BPA, DEHP and PBDE 99 are able to alter luteal steroidogenesis in bovine cells and can disrupt hormonal balance in the ovary. However, it is necessary to evaluate the exact mechanism underlying these effects in future studies.

中文翻译：

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双酚A，邻苯二甲酸二乙基己酯和五溴代二苯醚99对培养的黄体细胞中类固醇合成的影响。

双酚A（BPA），邻苯二甲酸二乙基己基酯（DEHP）和五溴代二苯醚99（PBDE 99）是属于内分泌干扰化合物（EDC）的环境毒物。它们对各种生理系统，特别是人和动物的生殖系统产生不利影响。这项研究的目的是调查BPA，DEHP和PBDE 99对培养的黄体细胞中孕酮（P4）合成的影响。从黄体中体分离的牛黄体细胞暴露于不同浓度的BPA（1、3、10和30 µM），DEHP（1、3、10和30 µM）和PBDE 99（0.1、0.3、1和3 µM）在无血清培养基中培养48和96 hr。在所有剂量的BPA处理后48小时，黄体细胞中的P4水平下降；3、10和30 µM的DEHP；p  <.05）。用3–30 µM BPA，1–30 µM DEHP和1–3 µM PBDE 99处理细胞96小时会导致P4合成减少（p  <.05）。但是，较低浓度的PBDE 99（0.1和0.3 µM）会在48和96小时增加P4水平。在用BPA（30 µM），DEHP（1–30 µM），PBDE 99（0.3–3 µM）和对照组治疗的48小时中，P4的合成在48小时时较低。我们的结果表明，BPA，DEHP和PBDE 99能够改变牛细胞中的黄体类固醇生成，并能破坏卵巢中的激素平衡。但是，有必要在以后的研究中评估这些效应的确切机制。