Here, we examined the efficacy of are combinant subunit antigen-based oral vaccine for preventing porcine epidemic diarrhea virus (PEDV). First, we generated a soluble recombinant partial spike S1 protein (aP2) from PEDV in E. coli and then evaluated the utility of aP2 subunit vaccine-loaded hydroxypropyl methylcellulose phthalate microspheres (HPMCP) and RANKL-secreting L. lactis (LLRANKL) as a candidate oral vaccine in pregnant sows. Pregnant sows were vaccinated twice (with a 2 week interval between doses) at 4 weeks before farrowing. Titers of virus-specific IgA antibodies in colostrum, and neutralizing antibodies in serum, of sows vaccinated with HPMCP (aP2) plus LL RANKL increased significantly at 4 weeks post-first vaccination. Furthermore, the survival rate of newborn suckling piglets delivered by sows vaccinated with HPMCP (aP2) plus LL RANKL was similar to that of piglets delivered by sows vaccinated with a commercial killed porcine epidemic diarrhea virus (PED) vaccine. The South Korean government promotes a PED vaccine program (live-killed-killed) to increase the titers of IgA and IgG antibodies in pregnant sows and prevent PEDV. The oral vaccine strategy described herein, which is based on a safe and efficient recombinant subunit antigen, is an alternative PED vaccination strategy that could replace the traditional strategy, which relies on attenuated live oral vaccines or artificial infection with virulent PEDV.

中文翻译：

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口服猪流行性腹泻疫苗的羟丙基甲基纤维素邻苯二甲酸酯微球和分泌RANKL的乳酸乳球菌的功效。

在这里，我们检查了基于亚单位联合抗原的口服疫苗预防猪流行性腹泻病毒（PEDV）的功效。首先，我们从大肠杆菌中的PEDV产生了可溶性重组部分穗S1蛋白（aP2），然后评估了载有aP2亚基疫苗的羟丙基甲基纤维素邻苯二甲酸酯微球（HPMCP）和分泌RANKL的乳酸乳球菌（LLRANKL）的效用。怀孕母猪的候选口服疫苗。在分娩前的第4周给怀孕的母猪接种两次疫苗（两次间隔两次）。初次接种疫苗后4周，用HPMCP（aP2）和LL RANKL接种的母猪初乳中的病毒特异性IgA抗体滴度和血清中和抗体的滴度显着增加。此外，用HPMCP（aP2）和LL RANKL疫苗接种的母猪提供的新生乳猪的存活率与用商品化的猪流行性腹泻病毒（PED）疫苗接种的母猪提供的仔猪的存活率相似。韩国政府推行了一项PED疫苗计划（活杀灭），以提高怀孕母猪的IgA和IgG抗体的滴度并预防PEDV。本文所述的基于安全有效的重组亚基抗原的口服疫苗策略是一种替代性PED疫苗接种策略，可以替代传统策略，后者依靠减毒的口服活疫苗或强毒PEDV人工感染。韩国政府推行了一项PED疫苗计划（活死杀），以增加怀孕母猪的IgA和IgG抗体的滴度并预防PEDV。本文描述的基于安全有效的重组亚基抗原的口服疫苗策略是一种替代性PED疫苗接种策略，可以替代传统策略，后者依赖于减毒的口服活疫苗或强毒PEDV的人工感染。韩国政府推行了一项PED疫苗计划（活杀灭），以提高怀孕母猪的IgA和IgG抗体的滴度并预防PEDV。本文描述的基于安全有效的重组亚基抗原的口服疫苗策略是一种替代性PED疫苗接种策略，可以替代传统策略，后者依赖于减毒的口服活疫苗或强毒PEDV的人工感染。