Pigeon circovirus (PiCV) is the most diagnosed virus in pigeons (Columba livia) and have been studied and reported globally. PiCV infections can lead to immunosuppression and pigeons infected with PiCV can result to lymphocyte apoptosis and atrophy of immune organs. Young pigeon disease syndrome (YPDS) is a complex disease and believed that PiCV could be one of the agents leading to this syndrome. An effective treatment regimen is needed to control the spread of PiCV in pigeons. In this study pigeon interferon alpha (PiIFN-α) was cloned and expressed and its antiviral effects were tested against fowl adenovirus type 4 (FAdV-4) in vitro and PiCV in vivo. No detectable levels of FAdV-4 viral genome in LMH cells stimulated with 300 μg/mL PiIFN-α were found. Additionally, PiIFN-α was stable at different temperature and pH for 4 h, and no reduction in antiviral activity was observed in untreated and treated cells. In pigeons naturally and experimentally infected by PiCV, no detectable levels of PiCV virus titers were found after treatment with PiIFN-α. Cytokine and ISG expression levels in liver and spleen samples were detected and IFN-γ and Mx1 genes were dominantly up-regulated following PiIFN-α treatment (p < 0.05). This study demonstrated that PiCV can be inhibited by administration of PiIFN-α and PiFN-α can be used as a therapeutic approach to prevent the spread of PiCV in pigeons.

中文翻译：

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鸽子干扰素α（PiIFN-α）对哥伦巴百合中的鸽子圆环病毒（PiCV）复制和细胞因子表达的影响。

鸽子圆环病毒（PiCV）是鸽子（Columba livia）中诊断最多的病毒，并且已在全球进行研究和报道。PiCV感染可导致免疫抑制，鸽子被PiCV感染可导致淋巴细胞凋亡和免疫器官萎缩。幼鸽疾病综合症（YPDS）是一种复杂的疾病，相信PiCV可能是导致这种综合症的病原之一。需要有效的治疗方案来控制PiCV在鸽子中的传播。在这项研究中，克隆并表达了鸽子干扰素α（PiIFN-α），并在体外和体内对4型禽腺病毒（FAdV-4）进行了抗病毒作用测试。在300μg/ mLPiIFN-α刺激的LMH细胞中，未检测到FAdV-4病毒基因组水平。此外，PiIFN-α在不同温度和pH下稳定4小时，在未处理和处理的细胞中未观察到抗病毒活性降低。在用PiCV自然感染和实验感染的鸽子中，用PiIFN-α治疗后未发现可检测到的水平的PiCV病毒滴度。在PiIFN-α治疗后，检测到肝和脾样品中的细胞因子和ISG表达水平，并且IFN-γ和Mx1基因显着上调（p <0.05）。这项研究表明，通过施用PiIFN-α可以抑制PiCV，PiFN-α可以用作预防PiCV在鸽子中传播的治疗方法。在PiIFN-α治疗后，检测到肝和脾样品中的细胞因子和ISG表达水平，并且IFN-γ和Mx1基因显着上调（p <0.05）。这项研究表明，通过施用PiIFN-α可以抑制PiCV，PiFN-α可以用作预防PiCV在鸽子中传播的治疗方法。在PiIFN-α治疗后，检测到肝和脾样品中的细胞因子和ISG表达水平，并且IFN-γ和Mx1基因显着上调（p <0.05）。这项研究表明，通过施用PiIFN-α可以抑制PiCV，PiFN-α可以用作预防PiCV在鸽子中传播的治疗方法。