SummaryIt is widely accepted that the oocyte plays a very active role in promoting the growth of the follicle by directing the differentiation of granulosa cells and secreting paracrine growth factors. In turn, granulosa cells regulate the development of the oocytes, establishing close bidirectional communication between germ and somatic cells. The presence of cortical cells with morphological characteristics, similar to primordial germ cells that express specific germline markers, stem cells and cell proliferation, known as adult cortical germ cells (ACGC) have been reported in phyllostomid bats. Using magnetic cell separation techniques, dissociation–cellular re-aggregation and organ culture, the behaviour of oocytes and ACGC was analyzed by interacting in vitro with mouse ovarian cells. Bat ACGC was mixed with disaggregated ovaries from a transgenic mouse that expressed green fluorescent protein. The in vitro reconstruction of the re-aggregates was evaluated. We examined the viability, integration, cellular interaction and ovarian morphogenesis by detecting the expression of Vasa, pH3, Cx43 and Laminin. Our results showed that the interaction between ovarian cells is carried out in the adult ovary of two species, without them losing their capacity to form follicular structures, even after having been enzymatically dissociated.

中文翻译：

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成年卵巢分离-再聚集后，小鼠和蝙蝠的卵母细胞、皮质生殖细胞和颗粒细胞之间的相互作用

总结人们普遍认为，卵母细胞通过指导颗粒细胞的分化和分泌旁分泌生长因子，在促进卵泡生长方面发挥着非常积极的作用。反过来，颗粒细胞调节卵母细胞的发育，在生殖细胞和体细胞之间建立密切的双向通讯。据报道，叶口蝠中存在具有形态特征的皮质细胞，类似于表达特定生殖系标记、干细胞和细胞增殖的原始生殖细胞，称为成年皮质生殖细胞 (ACGC)。使用磁性细胞分离技术、解离-细胞重新聚集和器官培养，通过相互作用分析卵母细胞和 ACGC 的行为体外与小鼠卵巢细胞。Bat ACGC 与来自表达绿色荧光蛋白的转基因小鼠的分解卵巢混合。这体外评估了重新聚集体的重建。我们通过检测 Vasa、pH3、Cx43 和层粘连蛋白的表达来检查活力、整合、细胞相互作用和卵巢形态发生。我们的研究结果表明，卵巢细胞之间的相互作用是在两个物种的成年卵巢中进行的，即使在酶解后，它们也不会失去形成卵泡结构的能力。